Shreffler Wayne G, Charlop-Powers Zachary, Sicherer Scott H
The Elliot and Roslyn Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Mount Sinai School of Medicine, New York, New York, USA.
Ann Allergy Asthma Immunol. 2006 Jun;96(6):865-9. doi: 10.1016/S1081-1206(10)61351-8.
Peanut allergy is a common and severe phenotype of food allergy with a strong genetic component; HLA class II polymorphisms are attractive candidate genes for this disorder.
To determine possible genotypic associations of HLA class II with peanut allergy and attempt replication of previously reported associations.
Sibling pairs discordant for peanut allergy were genotyped (low resolution) by polymerase chain reaction-based methods to 7 DQ and 18 DR allele groups. A chi2 analysis was undertaken against sibling controls with statistical adjustment for multiple analyses.
Seventy-three children with confirmed peanut allergy (mean age, 6.5 years; male, 72%; asthma, 58%; atopic dermatitis, 62%; allergic rhinitis, 67%; other food allergies, 41%) and 75 of their siblings who eat peanut (mean age, 8 years; male, 52%; asthma, 12%; atopic dermatitis, 22%; allergic rhinitis, 37%; other food allergy, 7%) were genotyped. Distribution of DQ7 (29% of children with peanut allergy vs 47% sibling controls) was statistically significantly different (P = .04) before statistical correction for multiple comparisons was made by multiplying them by the number of alleles tested (and not statistically significant after correction; P = .30). Distribution of DR11 was nearly statistically significant without statistical adjustment (26% with peanut allergy vs 41% of sibling controls; P = .07; corrected P = 1.3). Alleles that were previously reported to have a weak association with peanut allergy (DRB1 *03, *08; DQB1 *0302, *04) were not verified in this cohort (unadjusted P > .44).
We could not establish an association between the HLA class II alleles evaluated in this cohort of sibling pairs discordant for peanut allergy.
花生过敏是食物过敏中一种常见且严重的表型，具有很强的遗传因素；人类白细胞抗原（HLA）II类多态性是该疾病颇具吸引力的候选基因。
确定HLA II类基因与花生过敏之间可能的基因型关联，并尝试重复先前报道的关联。
采用基于聚合酶链反应的方法对花生过敏不一致的同胞对进行基因分型（低分辨率），检测7个DQ和18个DR等位基因组。对同胞对照进行卡方分析，并对多次分析进行统计调整。
对73名确诊花生过敏儿童（平均年龄6.5岁；男性占72%；哮喘占58%；特应性皮炎占62%；过敏性鼻炎占67%；其他食物过敏占41%）及其75名食用花生的同胞（平均年龄8岁；男性占52%；哮喘占12%；特应性皮炎占22%；过敏性鼻炎占37%；其他食物过敏占7%）进行基因分型。在对多次比较进行统计学校正之前（通过将其乘以检测的等位基因数量），DQ7的分布在统计学上有显著差异（花生过敏儿童中占29%，同胞对照中占47%；P = 0.04），校正后无统计学意义（P = 0.30）。DR11的分布在未进行统计学调整时接近有统计学意义（花生过敏者中占26%，同胞对照中占41%；P = 0.07；校正后P = 1.3）。先前报道与花生过敏有弱关联的等位基因（DRB1 *03、*08；DQB1 *0302、*04）在该队列中未得到验证（未校正P > 0.44）。
在这一花生过敏不一致的同胞对队列中，我们未能确定所评估的HLA II类等位基因之间存在关联。
