Smyczynski Cybelle, Roudier François, Gissot Lionel, Vaillant Emilie, Grandjean Olivier, Morin Halima, Masson Thimoté, Bellec Yannick, Geelen Danny, Faure Jean-Denis
Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, Route de St. Cyr, 78026 Versailles Cedex, France.
J Biol Chem. 2006 Sep 1;281(35):25475-84. doi: 10.1074/jbc.M601815200. Epub 2006 Jun 27.
PASTICCINO1 (PAS1) is a high molecular weight FK506-binding protein (FKBP) involved in the control of cell proliferation and differentiation during plant development. Mutations in the C-terminal region of PAS1 result in severe developmental defects. We show here that the C-terminal domain of PAS1 controls the subcellular distribution of this protein. We also demonstrated in vitro and in vivo, by Forster resonance energy transfer, that this C-terminal region is required for interaction with FAN (FKBP-associated NAC), a new member of the plant-specific family of NAC transcription factors. PAS1 and FAN are translocated into the nucleus upon auxin treatment in plant seedlings. The nuclear translocation of PAS1 is dependent on the presence of the C terminus of the protein. Finally, we showed that FAN is involved in PAS1-regulated processes because FAN overproduction partly complemented the pas1 phenotype. We suggest that PAS1 regulates the function of this NAC-like transcription factor by controlling its targeting to the nucleus upon plant cell division.
PASTICCINO1（PAS1）是一种高分子量FK506结合蛋白（FKBP），参与植物发育过程中细胞增殖和分化的调控。PAS1 C末端区域的突变会导致严重的发育缺陷。我们在此表明，PAS1的C末端结构域控制该蛋白的亚细胞分布。我们还通过福斯特共振能量转移在体外和体内证明，该C末端区域是与FAN（FKBP相关NAC）相互作用所必需的，FAN是植物特异性NAC转录因子家族的一个新成员。在植物幼苗中，生长素处理后PAS1和FAN会转运到细胞核中。PAS1的核转运依赖于该蛋白C末端的存在。最后，我们表明FAN参与PAS1调控的过程，因为FAN的过量表达部分互补了pas1表型。我们认为，PAS1通过在植物细胞分裂时控制该类NAC转录因子靶向细胞核来调节其功能。
