Luan T G, Yu Keith S H, Zhong Y, Zhou H W, Lan C Y, Tam Nora F Y
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen (Zhongshan) University, 135 Xiangangxi Road, Guangzhou 510275, PR China.
Chemosphere. 2006 Dec;65(11):2289-96. doi: 10.1016/j.chemosphere.2006.05.013. Epub 2006 Jun 27.
The PAH metabolites produced during degradation of fluorene, phenanthrene and pyrene by a bacterial consortium enriched from mangrove sediments were analyzed using the on-fiber silylation solid-phase microextraction (SPME) combining with gas chromatography-mass spectrometry (GC-MS) method. Seventeen metabolites at trace levels were identified in different PAH degradation cultures based on the full scan mass spectra. In fluorene degradation cultures, 1-, 2-, 3- and 9-hydroxyfluorene, fluorenone, and phthalic acid were detected. In phenanthrene and pyrene degradation cultures, various common metabolites such as phenanthrene and pyrene dihydrodiols, mono-hydroxy phenanthrene, dihydroxy pyrene, lactone and 4-hydroxyphenanthrene, methyl ester, and phthalic acid were found. The detection of various common and novel metabolites demonstrates that SPME combining with GC-MS is a quick and convenient method for identification as well as monitoring the real time changes of metabolite concentrations throughout the degradation processes. The knowledge of PAH metabolic pathways and kinetics within indigenous bacterial consortium enriched from mangrove sediments contributes to enhance the bioremediation efficiency of PAH in real environment.
采用纤维上硅烷化固相微萃取（SPME）结合气相色谱 - 质谱联用（GC - MS）方法，分析了从红树林沉积物中富集的细菌群落对芴、菲和芘进行降解过程中产生的多环芳烃（PAH）代谢产物。基于全扫描质谱，在不同的PAH降解培养物中鉴定出了17种痕量水平的代谢产物。在芴降解培养物中，检测到了1 - 、2 - 、3 - 和9 - 羟基芴、芴酮和邻苯二甲酸。在菲和芘降解培养物中，发现了各种常见的代谢产物，如菲和芘的二氢二醇、单羟基菲、二羟基芘、内酯以及4 - 羟基菲甲酯和邻苯二甲酸。各种常见和新型代谢产物的检测表明，SPME与GC - MS联用是一种快速便捷的方法，可用于鉴定以及监测整个降解过程中代谢产物浓度的实时变化。了解从红树林沉积物中富集的本地细菌群落内PAH的代谢途径和动力学，有助于提高实际环境中PAH的生物修复效率。
