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细胞穿透性肽核酸的合成及其杂交和摄取特性的表征。

Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties.

作者信息

Zhou Peng, Dragulescu-Andrasi Anca, Bhattacharya Birendra, O'Keefe Heather, Vatta Paolo, Hyldig-Nielsen Jens J, Ly Danith H

机构信息

Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.

出版信息

Bioorg Med Chem Lett. 2006 Sep 15;16(18):4931-5. doi: 10.1016/j.bmcl.2006.06.052. Epub 2006 Jun 30.

DOI:10.1016/j.bmcl.2006.06.052
PMID:16809033
Abstract

Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER).

摘要

已合成了含所有四种天然（腺嘌呤（A）、胞嘧啶（C）、鸟嘌呤（G）和胸腺嘧啶（T））以及两种非天然（2-硫尿嘧啶（sU）和2,6-二氨基嘌呤（D））核碱基的胍基肽核酸（GPNA）单体和寡聚物。热变性研究表明，含有交替D-主链构型的GPNA寡聚物与DNA序列特异性结合，并且在与哺乳动物细胞一起孵育时，特异性定位于内质网（ER）。

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