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肽核酸靶向前体微小RNA：干扰微小RNA成熟的新策略。

Targeting pre-miRNA by peptide nucleic acids: a new strategy to interfere in the miRNA maturation.

作者信息

Avitabile Concetta, Saviano Michele, D'Andrea Luca, Bianchi Nicoletta, Fabbri Enrica, Brognara Eleonora, Gambari Roberto, Romanelli Alessandra

机构信息

Dipartimento delle Scienze Biologiche, Università di Napoli "Federico II", Napoli, Italy.

出版信息

Artif DNA PNA XNA. 2012 Apr-Jun;3(2):88-96. doi: 10.4161/adna.20911. Epub 2012 Apr 1.

DOI:10.4161/adna.20911
PMID:22699795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3429535/
Abstract

PNAs conjugated to carrier peptides have been employed for the targeting of miRNA precursor, with the aim to develop molecules able to interfere in the pre-miRNA processing. The capability of the molecules to bind pre-miRNA has been tested in vitro by fluorescence assayes on Thiazole Orange labeled molecules and in vivo, in K562 cells, evaluating the amount of miRNA produced after treatment of cells with two amounts of PNAs.

摘要

与载体肽偶联的肽核酸已被用于靶向微小RNA前体，目的是开发能够干扰前体微小RNA加工的分子。这些分子与前体微小RNA结合的能力已通过对噻唑橙标记分子进行荧光测定在体外进行了测试，并在体内对K562细胞进行了测试，评估了用两种剂量的肽核酸处理细胞后产生的微小RNA的量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/5f00796fd9e4/adna-3-88-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/36869cb6621b/adna-3-88-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/ec2d60f47485/adna-3-88-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/7cbe65b6c8cf/adna-3-88-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/354916dc1641/adna-3-88-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/5f00796fd9e4/adna-3-88-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/36869cb6621b/adna-3-88-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/ec2d60f47485/adna-3-88-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/7cbe65b6c8cf/adna-3-88-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/354916dc1641/adna-3-88-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d6/3429535/5f00796fd9e4/adna-3-88-g5.jpg

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Nucleic Acids Res. 2012 Feb;40(4):1424-37. doi: 10.1093/nar/gkr903. Epub 2011 Nov 3.
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