Fujitani H, Holoubek V
Int J Cancer. 1975 Aug 15;16(2):329-38. doi: 10.1002/ijc.2910160217.
Nuclear proteins of rat liver and rat ascites hepatoma were fractionated by extraction in solutions of different salt concentration and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The difference between the electrophorograms of the different fractions of nuclear proteins isolated from liver and from hepatoma was found in the bands which have the same electrophoretic mobility as the main proteins of informofers and are extracted from nuclei at salt concentrations which extract informofers. These changes in the electrophoretic patterns of proteins with the solubility and mobility of the proteins of informofers could be related to the defective processing of heterogeneous nuclear RNA in the hepatoma. In addition the identity of electrophorograms of nuclear proteins isolated from liver and from hepatoma and the identity of most bands in the electrophorograms of nuclear proteins which are soluble in 0.35 M NaCl and chromosomal proteins which are not soluble at this salt concentration support the notion that these nonhistone nuclear proteins which can be identified as the major bands in electrophorograms of chromosomal proteins are not the specific regulators of gene expression.
通过在不同盐浓度的溶液中进行提取，对大鼠肝脏和大鼠腹水肝癌的核蛋白进行分级分离，并采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行分析。从肝脏和肝癌中分离出的不同级分核蛋白的电泳图谱差异，出现在那些与信息体主要蛋白质具有相同电泳迁移率、且在能提取信息体的盐浓度下从细胞核中提取出来的条带中。蛋白质电泳图谱的这些变化以及信息体蛋白质的溶解性和迁移率，可能与肝癌中核不均一RNA的加工缺陷有关。此外，从肝脏和肝癌中分离出的核蛋白电泳图谱的一致性，以及可溶于0.35M NaCl的核蛋白电泳图谱中大多数条带与在此盐浓度下不溶的染色体蛋白电泳图谱的一致性，支持了这样一种观点，即这些可被鉴定为染色体蛋白电泳图谱中主要条带的非组蛋白核蛋白并非基因表达的特异性调节因子。
