一种基因编码的荧光氨基酸。
A genetically encoded fluorescent amino acid.
作者信息
Wang Jiangyun, Xie Jianming, Schultz Peter G
机构信息
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
出版信息
J Am Chem Soc. 2006 Jul 12;128(27):8738-9. doi: 10.1021/ja062666k.
The fluorescent amino acid l-(7-hydroxycoumarin-4-yl) ethylglycine 1 has been genetically encoded in E. coli in response to the amber TAG codon. Because of its high fluorescence quantum yield, relatively large Stoke's shift, and sensitivity to both pH and polarity, this amino acid should provide a useful probe of protein localization and trafficking, protein conformation changes, and protein-protein interactions.
荧光氨基酸1-（7-羟基香豆素-4-基）乙基甘氨酸1已通过基因编码在大肠杆菌中响应琥珀色TAG密码子。由于其高荧光量子产率、相对较大的斯托克斯位移以及对pH值和极性的敏感性，这种氨基酸应能为蛋白质定位和运输、蛋白质构象变化以及蛋白质-蛋白质相互作用提供有用的探针。
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