Department of Environmental Analysis, Institute for Environmental and Human Health Protection, Faculty of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdańsk, Poland.
Z Naturforsch C J Biosci. 2013 May-Jun;68(5-6):210-22. doi: 10.1515/znc-2013-5-607.
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been applied for the first time to the analysis of the sucrose esters from the surface of Nicotiana L. leaves. The profiles obtained for the model plant N. tabacum were similar to those from the gas chromatography-flame ionization detector (GC-FID) analysis. The most reproducible results were obtained using a dihydroxybenzoic acid (DHB) matrix. The main advantage of this method is that crude plant extracts can be analysed without sample clean-up. GC-MS analysis of Aztec tobacco (N. rustica) extracts revealed the presence of three types of sucrose esters. All identified compounds had three C4-C8 acyl chains substituting the glucose moiety, while the fructose part of the molecule was substituted with 0, 1, or 2 acetyl groups. MALDI-TOF MS analysis of the sucrose ester fraction revealed the presence of compounds not eluting from a GC column. Combining the data from both GC-MS and MALDI-TOF MS experiments, we obtained a full sucrose ester profile, which is based on the molecular weight of the compounds and on the number of acyl chains in the molecule.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）首次被应用于分析烟草原生质体表面的蔗糖酯。模式植物烟草（Nicotiana tabacum）的分析图谱与气相色谱-火焰离子化检测器（GC-FID）的分析图谱相似。使用二羟基苯甲酸（DHB）基质可获得最具重现性的结果。该方法的主要优点是无需样品净化即可分析粗提植物提取物。GC-MS 分析阿兹特克烟草（Nicotiana rustica）提取物表明存在三种类型的蔗糖酯。所有鉴定出的化合物均具有三个取代葡萄糖部分的 C4-C8 酰基链，而分子的果糖部分则被 0、1 或 2 个乙酰基取代。MALDI-TOF MS 分析蔗糖酯馏分显示存在未从 GC 柱洗脱的化合物。结合 GC-MS 和 MALDI-TOF MS 实验数据，我们获得了基于化合物分子量和分子中酰基链数的完整蔗糖酯图谱。
