Langehanenberg Patrik, Kemper Björn, Dirksen Dieter, von Bally Gert
Center for Biomedical Optics and Photonics, University Medical Center of Muenster, Muenster, Germany.
Appl Opt. 2008 Jul 1;47(19):D176-82. doi: 10.1364/ao.47.00d176.
Digital holography enables a multifocus quantitative phase microscopy for the investigation of reflective surfaces and for marker-free live cell imaging. For digital holographic long-term investigations of living cells an automated (subsequent) robust and reliable numerical focus adjustment is of particular importance. Four numerical methods for the determination of the optimal focus position in the numerical reconstruction and propagation of the complex object waves of pure phase objects are characterized, compared, and adapted to the requirements of digital holographic microscopy. Results from investigations of an engineered surface and human pancreas tumor cells demonstrate the applicability of Fourier-weighting- and gradient-operator-based methods for robust and reliable automated subsequent numerical digital holographic focusing.
数字全息术实现了一种用于研究反射表面和无标记活细胞成像的多焦点定量相显微镜。对于活细胞的数字全息长期研究，自动（后续）稳健且可靠的数值聚焦调整尤为重要。对用于确定纯相位物体复物波数值重建和传播中最佳聚焦位置的四种数值方法进行了表征、比较，并使其适应数字全息显微镜的要求。对工程表面和人胰腺肿瘤细胞的研究结果表明，基于傅里叶加权和梯度算子的方法适用于稳健且可靠的自动后续数值数字全息聚焦。
