University of Muenster, Center for Biomedical Optics and Photonics, Robert-Koch-Str. 45, D-48149 Muenster, GermanyUniversity of Muenster, Gastroenterological Molecular Cell Biology, Department of Medicine B, D-48149 Muenster, Germany.
J Biomed Opt. 2011 Feb;16(2):026014. doi: 10.1117/1.3540674.
Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.
许多基于干涉的定量相位对比成像技术需要单独生成相干参考波。这导致相位稳定性低，并且需要精确调整物波和参考波之间的强度比。为了克服这些问题，分析了一种避免通过不同图像区域的叠加来单独生成参考波的数字全息显微镜中的 Michelson 干涉仪方法的性能。结果表明，这种简化的布置提高了相位稳定性。此外，对活胰腺肿瘤细胞的时程研究结果表明，该方法具有可靠的定量相位对比成像能力。
