Lu Baiyi, Wu Xiaoqin, Shi Jiayi, Dong Yuejie, Zhang Ying
Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, Zhejiang Province, PR China.
Food Chem Toxicol. 2006 Oct;44(10):1739-43. doi: 10.1016/j.fct.2006.05.012. Epub 2006 Jun 3.
The antioxidant of bamboo leaves (AOB) is a kind of polyphenols-rich extract from bamboo leaves of the Phyllostachys Sieb. et Zucc. family. It has been certificated as a natural antioxidant by the Ministry of Health of the People's Republic of China in 2003, which has a warrant for use in edible oil, meat product, aquatic product and puffed food as a novel food additive. For safely using AOB, it was required to systemic safety evaluation studies. As part of an extensive program of safety evaluation studies, the traditional teratogenicity test and the reproduction study were conducted in Sprague-Dawley rats. In the traditional teratogenicity test, the treatment was well tolerated and no mortality occurred at doses of 0, 1.43, 2.87 and 4.30 g/kg bw per day; weight gain during gestation, food consumption, and food efficiency were similar in all groups; reproductive performance was not effected by the treatment; examination of the fetuses for external, visceral, and skeletal alterations did not reveal any fetotoxic, embryotoxic, or teratogenic effects of AOB. Therefore, a nominal dietary AOB level of 4.30 g/kg bw per day (The value of intended use and potential exposure to human is 860 mg/60 kg bw per day dependent on the period of the study.) was considered to be the no-observed-adverse-effect level following daily oral administration of AOB. The results of our studies indicate safety of AOB and support the use of AOB as a food additive.
竹叶抗氧化剂（AOB）是一种从刚竹属竹子竹叶中提取的富含多酚的提取物。2003年，它被中华人民共和国卫生部认证为天然抗氧化剂，作为一种新型食品添加剂，可用于食用油、肉制品、水产品和膨化食品。为安全使用AOB，需要进行系统的安全性评价研究。作为广泛安全性评价研究计划的一部分，在斯普拉格-道利大鼠中进行了传统致畸试验和生殖研究。在传统致畸试验中，在每日剂量为0、1.43、2.87和4.30 g/kg体重时，受试动物耐受性良好，无死亡发生；妊娠期间体重增加、食物消耗和食物利用率在所有组中相似；生殖性能不受该处理影响；对胎儿进行外部、内脏和骨骼改变检查未发现AOB有任何胎儿毒性、胚胎毒性或致畸作用。因此，每日口服AOB后，4.30 g/kg体重的名义膳食AOB水平（根据研究阶段，预期使用和人类潜在暴露值为860 mg/60 kg体重/天）被认为是未观察到不良反应水平。我们的研究结果表明AOB是安全的，并支持将AOB用作食品添加剂。
