Cao Guangtian, Yu Yang, Wang Huixian, Liu Jinsong, Zhang Xiping, Yu Yue, Li Zhanming, Zhang Yan, Yang Caimei
College of Standardisation, China Jiliang University, Hangzhou, China.
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang A & F University, Hangzhou, China.
Front Nutr. 2022 Mar 3;9:848532. doi: 10.3389/fnut.2022.848532. eCollection 2022.
The consumption of bamboo leaf flavonoids (BLFs) as novel dietary antioxidants has increased owing to their beneficial biological and pharmacological functions. This study assessed the effects of BLFs on antioxidant capacity, as well as caecal microbiota, serum metabolome, and health status. The model and the oral administration approach were used with four treatment groups (basal diet, basal diet with 20 mg bacitracin/kg, basal diet with 50 mg BLF/kg, and basal diet with 250 mg BLF/kg). Ultra-high-performance liquid chromatography triple-quadrupole mass spectrometry analysis indicated that vitexin, fumaric acid, orientin, isoorientin, and -coumaric acid were the predominant BLF components. From days 1 to 21, BLF increased the average daily gain and decreased the feed:gain of broilers. Moreover, BLF enhanced the serum antioxidant capacity and immune responses. Further, 16S rRNA sequencing showed that BLF modulated the caecal microbial community structure, which was dominated by Betaproteobacteriales, , , , , and in BLF broilers. Among the 22 identified serum metabolites in BLF broilers, sphinganine, indole-3-acetaldehyde retinol, choline, 4-methylthio-2-oxobutanoic acid, and -phenylalanine were recognised as biomarkers. In summary, BLFs appeared to modulate the caecal microbiome, alter the serum metabolome, and indirectly improve antioxidant capacity and health status.
由于竹叶黄酮（BLFs）具有有益的生物学和药理功能，其作为新型膳食抗氧化剂的消费量有所增加。本研究评估了BLFs对抗氧化能力、盲肠微生物群、血清代谢组和健康状况的影响。采用模型和口服给药方法，设置四个处理组（基础日粮、含20 mg杆菌肽/kg的基础日粮、含50 mg BLF/kg的基础日粮和含250 mg BLF/kg的基础日粮）。超高效液相色谱三重四极杆质谱分析表明，牡荆素、富马酸、东方蓼黄素、异东方蓼黄素和对香豆酸是BLF的主要成分。在第1至21天，BLF提高了肉鸡的平均日增重并降低了料重比。此外，BLF增强了血清抗氧化能力和免疫反应。进一步的16S rRNA测序表明，BLF调节了盲肠微生物群落结构，在BLF组肉鸡中，盲肠微生物群落以β-变形菌纲、、、、和为主。在BLF组肉鸡中鉴定出的22种血清代谢物中，鞘氨醇、吲哚-3-乙醛视黄醇、胆碱、4-甲硫基-2-氧代丁酸和苯丙氨酸被识别为生物标志物。总之，BLFs似乎调节了盲肠微生物组，改变了血清代谢组，并间接改善了抗氧化能力和健康状况。
