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丝状噬菌体fl和fd的核苷酸序列及基因组结构

Nucleotide sequence and genome organisation of filamentous bacteriophages fl and fd.

作者信息

Beck E, Zink B

出版信息

Gene. 1981 Dec;16(1-3):35-58. doi: 10.1016/0378-1119(81)90059-7.

DOI:10.1016/0378-1119(81)90059-7
PMID:6282703
Abstract

The DNA sequence of the filamentous phage F1, consisting of 6407 nucleotides, has been determined. When compared with the DNA sequence of the related filamentous phage fd (Beck et al., 1978), the f1 sequence is one nucleotide shorter and differs in 180 positions from the fd DNA. Only ten of these base exchanges cause amino acid exchanges in the known gene products. Most of the exchanges in f1 are the same as in M13 (Van Wezenbeek et al., 1980), showing a near identity of these two phage (there are only 59 nucleotide differences). Regulatory units for replication, transcription, and translation are in their essential parts identical in all three phage.

摘要

丝状噬菌体F1由6407个核苷酸组成的DNA序列已被测定。与相关丝状噬菌体fd的DNA序列(Beck等人,1978年)相比,F1序列少一个核苷酸,与fd DNA在180个位置上不同。这些碱基交换中只有十个会导致已知基因产物中的氨基酸交换。F1中的大多数交换与M13中的相同(Van Wezenbeek等人,1980年),表明这两种噬菌体几乎相同(只有59个核苷酸差异)。复制、转录和翻译的调控单元在所有三种噬菌体的基本部分是相同的。

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