Castillo A M, Gutiérrez M C, Kamekura M, Xue Y, Ma Y, Cowan D A, Jones B E, Grant W D, Ventosa A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.
Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan.
Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1519-1524. doi: 10.1099/ijs.0.64286-0.
Strain XH-48(T) was isolated from the sediment of Lake Xilinhot, a saline lake in Inner Mongolia (China). The organism is pleomorphic, neutrophilic and requires at least 2.5 M (15 %) NaCl, but not MgCl(2), for growth; it exhibits optimal growth at 3.4 M (20 %) NaCl. The G+C content of its DNA is 61 mol%. 16S rRNA gene sequence analysis revealed that strain XH-48(T) is a member of the family Halobacteriaceae, but there were low levels of similarity with other members of this family. The highest sequence similarity values (94.5 and 93.3 %) were obtained with the 16S rRNA genes of Natrialba aegyptiaca and Natrialba asiatica, respectively. Polar lipid analyses revealed that strain XH-48(T) contains phosphatidylglycerol and phosphatidylglyceromethylphosphate, derived from both C(20)C(20) and C(20)C(25) glycerol diethers, and two unidentified glycolipids. On the basis of the data obtained, the novel isolate cannot be classified within any recognized genus. Strain XH-48(T) should be placed within a novel genus and species within the family Halobacteriaceae, order Halobacteriales, for which the name Halostagnicola larsenii gen. nov., sp. nov. is proposed. The type strain of Halostagnicola larsenii is strain XH-48(T) (=DSM 17691(T)=CGMCC 1.5338(T)=JCM 13463(T)=CECT 7116(T)).
菌株XH-48(T)是从中国内蒙古的一个盐湖——锡林浩特湖的沉积物中分离得到的。该菌株具有多形态性，嗜中性，生长需要至少2.5 M（15%）的NaCl，但不需要MgCl₂；在3.4 M（20%）的NaCl浓度下生长最佳。其DNA的G+C含量为61 mol%。16S rRNA基因序列分析表明，菌株XH-48(T)是嗜盐菌科的成员，但与该科其他成员的相似性较低。与埃及嗜盐嗜盐碱杆菌（Natrialba aegyptiaca）和亚洲嗜盐嗜盐碱杆菌（Natrialba asiatica）的16S rRNA基因分别获得了最高的序列相似性值（94.5%和93.3%）。极性脂质分析表明，菌株XH-48(T)含有磷脂酰甘油和磷脂酰甘油甲基磷酸酯，它们来源于C₂₀C₂₀和C₂₀C₂₅甘油二醚，以及两种未鉴定的糖脂。根据所获得的数据，该新分离菌株不能归类于任何已被认可的属。菌株XH-48(T)应置于嗜盐菌科、嗜盐菌目内的一个新属和新种中，为此提出新名称拉氏嗜盐盐湖菌（Halostagnicola larsenii），属名新拟，种名新拟。拉氏嗜盐盐湖菌的模式菌株是菌株XH-48(T)（=DSM 17691(T)=CGMCC 1.5338(T)=JCM 13463(T)=CECT 7116(T)）。
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