Extremophile Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1021-1026. doi: 10.1099/ijs.0.033167-0. Epub 2011 Jun 17.
Strain EB21(T) was isolated from a brine sample from Aran-Bidgol salt lake, a saline playa in Iran. Strain EB21(T) was an orange-red-pigmented, motile rod and required at least 2 M NaCl but not MgCl(2) for growth. Optimal growth was achieved at 3.5 M NaCl and 0.2 M MgCl(2). The optimum pH and temperature for growth were pH 7.5 and 40 °C, while it was able to grow at pH 6.0-8.0 and 25-55 °C. Analysis of the 16S rRNA gene sequence revealed that strain EB21(T) is a member of the family Halobacteriaceae, showing low levels of similarity to other members of the family. The highest sequence similarities, 91.8, 91.7 and 91.5 %, were obtained with the 16S rRNA gene sequences of the type strains of Halobiforma lacisalsi, Haloterrigena thermotolerans and Halalkalicoccus tibetensis, respectively. Polar lipid analyses revealed that strain EB21(T) contains phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Three unidentified glycolipids and one minor phospholipid were also observed. The only quinone present was MK-8(II-H(2)). The G+C content of its DNA was 67.7 mol%. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain EB21(T) is thus considered to represent a novel species in a new genus within the family Halobacteriaceae, order Halobacteriales, for which the name Haloarchaeobius iranensis gen. nov., sp. nov. is proposed. The type strain of Haloarchaeobius iranensis is EB21(T) ( = IBRC-M 10013(T) = KCTC 4048(T)).
EB21(T) 菌株从伊朗盐水湖 Aran-Bidgol 盐沼的盐样中分离得到。EB21(T) 菌株为橙色红色、运动杆状，生长需要至少 2 M NaCl，但不需要 MgCl(2)。最佳生长条件为 3.5 M NaCl 和 0.2 M MgCl(2)。最佳生长的 pH 值和温度为 pH7.5 和 40°C，可在 pH6.0-8.0 和 25-55°C 下生长。16S rRNA 基因序列分析表明，EB21(T) 菌株是盐杆菌科的一个成员，与该科的其他成员相似度较低。与模式菌株 Halobiforma lacisalsi、Haloterrigena thermotolerans 和 Halalkalicoccus tibetensis 的 16S rRNA 基因序列相似度最高，分别为 91.8%、91.7%和 91.5%。极性脂分析表明，EB21(T) 菌株含有磷脂酰甘油、磷脂酰甘油磷酸甲基酯和磷脂酰甘油硫酸酯。还观察到三种未鉴定的糖脂和一种少量的磷脂。唯一存在的醌是 MK-8(II-H(2))。其 DNA 的 G+C 含量为 67.7 mol%。基于获得的数据，新分离株不能归入任何已识别的属。因此，EB21(T) 菌株被认为代表了盐杆菌科、盐杆菌目新属中的一个新种，建议将其命名为 Haloarchaeobius iranensis gen. nov.，sp. nov.。Haloarchaeobius iranensis 的模式菌株为 EB21(T)（ = IBRC-M 10013(T) = KCTC 4048(T)）。
