Department of Otorhinolaryngology, Ostersund Hospital, Ostersund, Sweden.
Laryngoscope. 2010 Jul;120(7):1370-5. doi: 10.1002/lary.20926.
OBJECTIVES/HYPOTHESIS: The aims were to analyze if improved histological and viscoelastic properties seen after injection of human mesenchymal stem cells (hMSCs) in scarred vocal folds (VFs) of rabbits are sustainable and if the injected hMSCs survive 3 months in the VFs.
Experimental xenograft model.
Eighteen VFs of 11 New Zealand white rabbits were scarred by a bilateral localized resection. After 3 months the animals were sacrificed. Twelve VFs were dissected and stained for histology, lamina propria thickness, and relative collagen type I analyses. The hMSCs survival was analyzed using a human DNA-specific reference probe, that is, fluorescence in situ hybridization staining. Viscoelasticity, measured as the dynamic viscosity and elastic modulus, was analyzed in a parallel-plate rheometer for 10 VFs.
The dynamic viscosity and elastic modulus of hMSC-treated VFs were similar to that of normal controls and significantly improved compared to untreated controls (P < .05). A reduction in lamina propria thickness and relative collagen type 1 content were also shown for the hMSC-treated VFs compared to the untreated VFs (P < .05). The histological pictures corresponded well to the viscoelastic results. No hMSCs survived.
Human mesenchymal stem cells injected into a scarred vocal fold of rabbit enhance healing of the vocal fold with reduced lamina propria thickness and collagen type I content and restore the viscoelastic function.
目的/假设：本研究旨在分析人骨髓间充质干细胞（hMSCs）注射到兔瘢痕性声带后的组织学和黏弹性改善是否可持续，以及注射的 hMSCs 是否能在声带中存活 3 个月。
实验性异种移植物模型。
11 只新西兰白兔的 18 个声带通过双侧局部切除术形成瘢痕。3 个月后处死动物。12 个声带被解剖并进行组织学、固有层厚度和相对 I 型胶原分析染色。hMSC 存活情况采用人 DNA 特异性参考探针（荧光原位杂交染色）进行分析。10 个声带采用平行板流变仪分析黏弹性，测量动态黏度和弹性模量。
hMSC 处理的声带的动态黏度和弹性模量与正常对照组相似，与未处理对照组相比显著改善（P <.05）。与未处理的声带相比，hMSC 处理的声带固有层厚度和相对 I 型胶原含量也降低（P <.05）。组织学图片与黏弹性结果相符。未检测到 hMSCs 存活。
将 hMSCs 注射到兔的瘢痕性声带中，可减轻固有层厚度和 I 型胶原含量，恢复黏弹性功能，从而增强声带的愈合。
