Fleming K K, Hubel A
Department of Mechanical Engineering, University of Minnesota, 1100 Mechanical Engineering, 111 Church Street SE, Minneapolis, MN 55455, USA.
Transfus Apher Sci. 2006 Jun;34(3):309-15. doi: 10.1016/j.transci.2005.11.012. Epub 2006 Jul 10.
Recent studies illustrate the potential for improving the cryopreservation of stem cells. Reduced DMSO concentrations in the cryopreservation medium, post thaw washing of cells and increased cell concentration have been actively studied. Standardization of cell processing has led to the study of liquid storage prior to cryopreservation, validation of mechanical (uncontrolled rate freezing) freezing, and cryopreservation bag failure. Finally, the need for the systematic study and optimization of preservation processes has not been fulfilled. As the sources and applications of stem cells (hematopoietic and non-hematopoietic) continue to be developed, the need for effective preservation methods will only grow.
近期研究表明了改善干细胞冷冻保存的潜力。人们积极研究了降低冷冻保存培养基中的二甲基亚砜（DMSO）浓度、细胞解冻后的洗涤以及提高细胞浓度。细胞处理的标准化引发了对冷冻保存前液体储存的研究、机械（非控制速率冷冻）冷冻的验证以及冷冻保存袋故障的研究。最后，对保存过程进行系统研究和优化的需求尚未得到满足。随着干细胞（造血干细胞和非造血干细胞）的来源和应用不断发展，对有效保存方法的需求只会增加。
