人单核细胞衍生的树突状细胞是几种补体蛋白的来源。
Human monocyte-derived dendritic cells are a source of several complement proteins.
作者信息
Reis E S, Barbuto J A M, Isaac L
机构信息
Laboratório de Complemento, Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1730, CEP 05508-900, São Paulo, SP, Brazil.
出版信息
Inflamm Res. 2006 May;55(5):179-84. doi: 10.1007/s00011-006-0068-y.
OBJECTIVE
Little is known about the role of local production of complement components by dendritic cells (DCs) during the generation of specific immune responses. In this study, we demonstrate that human DCs are an extrahepatic source of several soluble complement proteins.
METHODS
Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to evaluate the expression and production of several complement proteins.
RESULTS
We show that DCs produce C3, C5, C9, Factor (F)I, FH, FB, FD and properdin at levels similar to macrophages. Treatment of DCs with lipopolysaccharide (LPS) promoted an increase in the expression of C3 and FI mRNAs and a decrease in C5 mRNA, while C9, FH, FB, FD and properdin mRNA levels were not affected. Treatment with interleukin (IL) -1 or dexamethasone induced a modest increase in C3 mRNA levels and did not affect the expression of other complement components.
CONCLUSION
DCs are a source of complement proteins whose synthesis may be regulated in response to different inflammatory stimuli.
目的
关于树突状细胞（DCs）在特异性免疫反应产生过程中局部产生补体成分的作用，目前所知甚少。在本研究中，我们证明人类DCs是几种可溶性补体蛋白的肝外来源。
方法
采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法评估几种补体蛋白的表达和产生情况。
结果
我们发现DCs产生C3、C5、C9、因子（F）I、FH、FB、FD和备解素的水平与巨噬细胞相似。用脂多糖（LPS）处理DCs可促进C3和FI mRNA表达增加，C5 mRNA表达减少，而C9、FH、FB、FD和备解素mRNA水平不受影响。用白细胞介素（IL）-1或地塞米松处理可使C3 mRNA水平适度增加，且不影响其他补体成分的表达。
结论
DCs是补体蛋白的来源，其合成可能会根据不同的炎症刺激进行调节。
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