Goetz A Katrin, Scheffler Bjorn, Chen Huan-Xin, Wang Shanshan, Suslov Oleg, Xiang Hui, Brüstle Oliver, Roper Steve N, Steindler Dennis A
Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL 32610, USA.
Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):11063-8. doi: 10.1073/pnas.0510926103. Epub 2006 Jul 10.
It was, until now, not entirely clear how the nervous system attains its cellular phenotypic diversity and wired complexity during development. Here we describe how environmental interactions alone can modify the development of neurogenic precursor cells. Upon evaluating distinct growth-permissive substrates in an embryonic stem cell-neurogenesis assay, we found that laminin, fibronectin, and gelatin instruct neural fate and alter the functional specification of neurons when applied at distinct stages of development. Changes in phenotypic, electrophysiological, and molecular characteristics could resemble cellular events and interactions in the early embryonic brain and may explain why these extracellular matrix components transiently demarcate certain developing brain structures.
直到现在，神经系统在发育过程中如何获得其细胞表型多样性和有线复杂性仍不完全清楚。在这里，我们描述了仅环境相互作用如何能够改变神经源性前体细胞的发育。在胚胎干细胞神经发生试验中评估不同的生长允许底物时，我们发现层粘连蛋白、纤连蛋白和明胶在发育的不同阶段应用时，会指导神经命运并改变神经元的功能特化。表型、电生理和分子特征的变化可能类似于早期胚胎大脑中的细胞事件和相互作用，并且可以解释为什么这些细胞外基质成分会短暂地划定某些发育中的脑结构。
