Masters C I, Murray R G, Moseley B E, Minton K W
Department of Pathology, F. E. Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
J Gen Microbiol. 1991 Jul;137(7):1459-69. doi: 10.1099/00221287-137-7-1459.
Nineteen new Deinococcus isolates from soil, 18 of which were from samples immediately adjacent to a lake in Nottingham, UK, were characterized by conventional criteria, and found to be identical to each other and to conform most closely to the species D. radiopugnans. However, we detected three different restriction enzyme activities in three different isolates. Because of this suggestion of heterogeneity, we examined the isolates for restriction fragment length polymorphisms (RFLPs). RFLP analysis of the 19 original isolates, using three different probes, distinguished 17 divergent groups. This extraordinary diversity cannot be attributed to geographical differences, nor to the method of isolation, which employed UV-radiation selection.
从土壤中分离出19株新的嗜放射球菌，其中18株来自英国诺丁汉一个湖泊附近的样本，通过传统标准对其进行了表征，发现它们彼此相同，且与放射抗性嗜放射球菌物种最为相符。然而，我们在三个不同的分离株中检测到了三种不同的限制酶活性。由于存在这种异质性的迹象，我们对这些分离株进行了限制性片段长度多态性（RFLP）检测。使用三种不同的探针对这19株原始分离株进行RFLP分析，区分出了17个不同的组。这种非同寻常的多样性既不能归因于地理差异，也不能归因于采用紫外线辐射选择的分离方法。
