Eyles R F, Brooks H J L, Townsend C R, Burtenshaw G A, Heng N C K, Jack R W, Weinstein P
Ecology and Health Research Centre, Department of Public Health, Wellington School of Medicine, University of Otago, Wellington, New Zealand.
J Appl Microbiol. 2006 Jul;101(1):18-25. doi: 10.1111/j.1365-2672.2006.02945.x.
To determine the degree of overlap in strain types of Campylobacter jejuni isolated from clinical cases and water samples from the Taieri catchment in the South Island of New Zealand.
Thermophilic Campylobacter were collected from human cases of infection, the main stem of the Taieri River and streams within distinct land-use types over a 1-year period. Campylobacter jejuni (187 isolates) and Campylobacter lari (four isolates) were identified using a multiplex polymerase chain reaction protocol. Isolates were typed by the Penner method and pulsed-field gel electrophoresis (PFGE) utilizing two restriction endonucleases. Several serotypes and PFGE types occurred in both water samples and clinical cases when the restriction profiles for each enzyme were considered separately. However, when PFGE profiles and serotyping were combined, there was no overlap between Camp. jejuni types from water and clinical cases.
The results of this study indicate that recreational water in the Taieri catchment is not a major source of campylobacteriosis in the Dunedin area.
This study suggests the risk of acquiring campylobacteriosis from surface waters in the Taieri catchment is considerably lower than previously predicted and highlights the necessity of using two endonucleases in PFGE typing.
确定从新西兰南岛泰里集水区的临床病例和水样中分离出的空肠弯曲菌菌株类型的重叠程度。
在1年时间内，从人类感染病例、泰里河主干以及不同土地利用类型内的溪流中收集嗜热弯曲菌。使用多重聚合酶链反应方案鉴定出空肠弯曲菌（187株分离株）和海鸥弯曲菌（4株分离株）。采用Penner方法和利用两种限制性内切酶的脉冲场凝胶电泳（PFGE）对分离株进行分型。当分别考虑每种酶的限制性图谱时，水样和临床病例中出现了几种血清型和PFGE型。然而，当将PFGE图谱和血清分型结合起来时，水样和临床病例中的空肠弯曲菌类型之间没有重叠。
本研究结果表明，泰里集水区的娱乐用水不是达尼丁地区弯曲菌病的主要来源。
本研究表明，从泰里集水区地表水感染弯曲菌病的风险远低于先前预测，并强调了在PFGE分型中使用两种限制性内切酶的必要性。
