日本曲霉-FCL 119T和黑曲霉ATCC 20611在生产低聚果糖时所使用的甘蔗 molasses和酵母粉。 注：molasses常见释义为“糖蜜”“糖浆”，这里直接保留英文未翻译，可能原文有误，正常应该是有准确释义的词汇。
Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611.
作者信息
Dorta Claudia, Cruz Rubens, de Oliva-Neto Pedro, Moura Danilo José Camargo
机构信息
Departamento de Ciências Biológicas-F.C.L., Universidade Estadual Paulista-UNESP, Assis, SP, Av. Dom Antônio, 2100, São Paulo, Brazil.
出版信息
J Ind Microbiol Biotechnol. 2006 Dec;33(12):1003-9. doi: 10.1007/s10295-006-0152-x. Epub 2006 Jul 12.
Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. These media were formulated for analysis of cellular growth, beta-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U ( t )) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains.
在米曲霉-FCL 119T和黑曲霉ATCC 20611的培养过程中，研究了不同浓度的蔗糖（3-25% w/v）和蛋白胨（2-5% w/v）在培养基配方中的作用。此外，甘蔗糖蜜（总糖含量为3.5-17.5% w/v）和酵母粉（1.5-5% w/v）被用作这两种菌株培养的替代营养物质。这些培养基用于分析细胞生长、β-果糖基转移酶和低聚果糖（FOS）的产生。通过高效液相色谱法分析转果糖基化活性（U（t））和FOS的产生。两种菌株产生酶的最高产量是在含有3%（w/v）蔗糖和3%（w/v）蛋白胨，或含有3.5%（w/v）甘蔗糖蜜中总糖以及1.5%（w/v）酵母粉的培养基中。在所研究的菌株中，甘蔗糖蜜和酵母粉在产生酶和FOS（约60% w/w）方面与蔗糖和蛋白胨一样好。
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