Fernandez Rubén Cuervo, Ottoni Cristiane Angélica, da Silva Elda Sabino, Matsubara Rosa Mitiko Saito, Carter José Márcio, Magossi Luis Roberto, Wada Maria Alice Alves, de Andrade Rodrigues Maria Filomena, Maresma Beatriz Guilarte, Maiorano Alfredo Eduardo
Instituto Cubano de Investigaciones Azucareras-ICINAZ, Carretera al Central Manuel M Prieto Km 21/2, Boyeros, Havana, Cuba.
Appl Microbiol Biotechnol. 2007 May;75(1):87-93. doi: 10.1007/s00253-006-0803-x. Epub 2007 Mar 21.
Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). In addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-beta-fructofuranosidase reaction rate were studied using a 2(2) factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V (t)/V (h) ratio. At 40 and 50 degrees C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.
在分批培养中培养了17种不同的丝状真菌菌株，以比较它们产生β-呋喃果糖苷酶的能力。其中三种，米曲霉IPT-301、黑曲霉ATCC 20611和菌株IPT-615，表现出高产，总果糖基转移酶活性高于12500单位l(-1)。此外，这些菌株的β-呋喃果糖苷酶具有高果糖基转移酶活性与水解活性之比。使用2(2)析因实验设计研究了温度和pH对蔗糖-β-呋喃果糖苷酶反应速率的影响。对测试变量系数的比较分析表明，变量pH对果糖基转移酶和水解速率以及V(t)/V(h)比值的变化贡献最大。在40和50℃时，这些酶的果糖基转移酶和水解速度之间没有显著差异。
