Microbial Technology Laboratory, Department of Zoology, Dr. Harisingh Gour University Sagar, 470003 MP, India.
Microbial Technology Laboratory, Department of Zoology, Dr. Harisingh Gour University Sagar, 470003 MP, India.
Carbohydr Polym. 2014 Sep 22;110:253-8. doi: 10.1016/j.carbpol.2014.03.066. Epub 2014 Apr 3.
Production of fructooligosaccharide (FOS) through efficient cultivation of biotransformation process by fructosyltransferase (FTase) was evaluated by two new isolates, Aspergillus niger and Aspergillus flavus NFCCI 2364. The saccharide consumption revealed lag phase of A. niger in 10 h which were smaller extent than A. flavus of 14 h. For the recycling of cell culture, the pellet cells were continuously reused after 24 h of submerged fermentation by these microorganisms in which FTase activity remains stable in four consecutive cycles in A. niger and six cycles in A. flavus. When freshly prepared pellets were sonicated for efficient release of intracellular FTase, the best transformation reaction was performed at 20 W acoustic power giving conversion yield of FOS 61.43% (w/w) by A. niger and 70.44% (w/w) by A. flavus respectively. This study was shown that the two fungal isolates can serve as veritable source of intracellular FTase for industrial production of FOS.
通过果糖基转移酶（FTase）的生物转化过程的高效培养，评估了两种新分离株黑曲霉和黄曲霉 NFCCI 2364 生产果寡糖（FOS）。糖的消耗表明黑曲霉的延滞期为 10 h，比黄曲霉的 14 h 小。为了进行细胞培养的回收，在微生物的 24 h 浸没发酵后，沉淀细胞可以连续重复使用，其中 FTase 活性在黑曲霉的连续 4 个循环和黄曲霉的 6 个循环中保持稳定。当新鲜制备的颗粒细胞经超声处理以有效释放细胞内 FTase 时，黑曲霉的最佳转化反应在 20 W 声功率下进行，转化率为 61.43%（w/w），黄曲霉的转化率为 70.44%（w/w）。本研究表明，这两种真菌分离株可以作为细胞内 FTase 的可靠来源，用于 FOS 的工业生产。
