Genç Mehmet R, Delaney Mary L, Onderdonk Andrew B, Witkin Steven S
Department of Obstetrics and Gynecology, Brigham & Women's Hospital, Boston, MA 02115, USA.
Am J Reprod Immunol. 2006 Aug;56(2):86-90. doi: 10.1111/j.1600-0897.2006.00388.x.
To evaluate vaginal nitric oxide (NO) production in response to alterations in the vaginal microbial flora.
Cervicovaginal lavage samples from 206 women at 18-22 weeks of gestation were tested for NO, interleukin-1beta (IL-1beta), IL-1 receptor antagonist (IL-1ra), tumor necrosis factor-alpha and the inducible 70 kDa heat shock protein (hsp70). Bacterial vaginosis (BV) was diagnosed based on gram staining of vaginal smears.
Elevated NO (>2.14 mmol/L) was associated with a diagnosis of BV (38% versus 11%, P < 0.008) as well as an increased median vaginal IL-1ra concentration (72.5 ng/ml versus 36.6 ng/ml, P = 0.041). Elevated vaginal NO was also associated with vaginal hsp70 and this relationship was independent of BV status or IL-1ra concentrations (P < 0.026). We conclude that vaginal hsp70 release in response to abnormal vaginal microflora may trigger NO production in an attempt to minimize the pathological consequences of this altered milieu.
评估阴道微生物群改变时阴道一氧化氮（NO）的产生情况。
对206名妊娠18 - 22周女性的宫颈阴道灌洗样本进行NO、白细胞介素-1β（IL-1β）、IL-1受体拮抗剂（IL-1ra）、肿瘤坏死因子-α和诱导型70 kDa热休克蛋白（hsp70）检测。根据阴道涂片革兰氏染色诊断细菌性阴道病（BV）。
NO升高（>2.14 mmol/L）与BV诊断相关（38%对11%，P < 0.008），同时阴道IL-1ra中位数浓度升高（72.5 ng/ml对36.6 ng/ml，P = 0.041）。阴道NO升高还与阴道hsp70相关，且这种关系独立于BV状态或IL-1ra浓度（P < 0.026）。我们得出结论，阴道hsp70因阴道微生物群异常而释放，可能会触发NO产生，以尽量减少这种改变的环境所带来的病理后果。
