孕期、分娩期以及细菌性阴道病患者阴道内炎性细胞因子的特征分析

Characterization of the inflammatory cytokines in the vagina during pregnancy and labor and with bacterial vaginosis.

作者信息

Imseis H M, Greig P C, Livengood C H, Shunior E, Durda P, Erikson M

机构信息

Department of Obstetrics and Gynecology, Duke University School of Medicine, Durham, North Carolina, USA.

出版信息

J Soc Gynecol Investig. 1997 Mar-Apr;4(2):90-4.

PMID:9101468

Abstract

OBJECTIVES

To characterize the presence of interleukin-1 alpha (IL-1 alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) in the vagina during pregnancy and in labor; 2) to compare the vaginal levels of these inflammatory cytokines between laboring and nonlaboring patients; and 3) to compare the vaginal levels of these cytokines between women with and without bacterial vaginosis (BV).

METHODS

Vaginal fluid was obtained by lavage from pregnant women with intact membranes at various gestational ages and during labor at term. These samples were analyzed for specific cytokine levels using standard enzyme-linked immunosorbent assay techniques. A Gram stain, wet mount, and pH were obtained from the vaginal fluid and were used to diagnose BV. The Mann-Whitney U test was used to evaluate the individual cytokine levels between groups, with P < .05 considered statistically significant.

RESULTS

There was a wide range of vaginal cytokine levels found in our pregnant population (N = 72). Vaginal levels of IL-1 beta (median 1070 versus 245.7 pg/mL) and IL-6 (9.0 versus 0 pg/mL) were found to be significantly elevated in laboring patients as compared with nonlaboring patients (P = .005 and P = .002, respectively). There were no significant differences in the levels of IL-1 alpha and TNF-alpha between the laboring and nonlaboring women. Interleukin-1 beta was also found to be significantly elevated in the group of patients with BV (3364 versus 245.7 pg/mL; P = .01), particularly those who were nonlaboring (P = .003). In each individual patient, there was a wide variation in the levels of the four different cytokines.

CONCLUSIONS

Measurable levels of the inflammatory cytokines IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha were present in the vagina during pregnancy and labor. Vaginal levels of IL-1 beta and IL-6 were found to be significantly elevated in laboring patients as compared with nonlaboring patients. Vaginal levels of IL-1 beta were also significantly elevated in nonlaboring patients with BV.

摘要

目的

1）描述孕期及分娩时阴道中白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的存在情况；2）比较分娩患者与未分娩患者这些炎性细胞因子的阴道水平；3）比较有无细菌性阴道病（BV）的女性这些细胞因子的阴道水平。

方法

通过灌洗获取不同孕周胎膜完整的孕妇及足月分娩时孕妇的阴道液。使用标准酶联免疫吸附测定技术分析这些样本中特定细胞因子的水平。从阴道液中获取革兰氏染色、湿片检查及pH值，用于诊断BV。采用曼-惠特尼U检验评估各组间单个细胞因子的水平，P < 0.05被认为具有统计学意义。

结果

在我们的孕妇群体（N = 72）中发现阴道细胞因子水平范围广泛。与未分娩患者相比，分娩患者的阴道IL-1β水平（中位数1070对245.7 pg/mL）和IL-6水平（9.0对0 pg/mL）显著升高（分别为P = 0.005和P = 0.002）。分娩女性与未分娩女性的IL-1α和TNF-α水平无显著差异。还发现BV患者组中IL-1β水平显著升高（3364对245.7 pg/mL；P = 0.01），尤其是未分娩患者（P = 0.003）。在每个个体患者中，四种不同细胞因子的水平存在很大差异。