在明显无性的尾孢菌属物种中进行的交配型基因分析表明存在隐秘的有性生殖。
Mating type gene analysis in apparently asexual Cercospora species is suggestive of cryptic sex.
作者信息
Groenewald Marizeth, Groenewald Johannes Z, Harrington Thomas C, Abeln Edwin C A, Crous Pedro W
机构信息
Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, Uppsalalaan 8, Utrecht, The Netherlands; Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, Wageningen, The Netherlands.
出版信息
Fungal Genet Biol. 2006 Dec;43(12):813-25. doi: 10.1016/j.fgb.2006.05.008. Epub 2006 Jul 12.
The genus Cercospora consists of numerous important, apparently asexual plant pathogens. We designed degenerate primers from homologous sequences in related species to amplify part of the C. apii, C. apiicola, C. beticola, C. zeae-maydis and C. zeina mating type genes. Chromosome walking was used to determine the full length mating type genes of these species. Primers were developed to amplify and sequence homologous portions of the mating type genes of additional species. Phylogenetic analyses of these sequences revealed little variation among members of the C. apii complex, whereas C. zeae-maydis and C. zeina were found to be dissimilar. The presence of both mating types in approximately even proportions in C. beticola, C. zeae-maydis and C. zeina populations, in contrast to single mating types in C. apii (MAT1) and C. apiicola (MAT2), suggests that a sexual cycle may be active in some of these species.
尾孢菌属包含众多重要的、显然为无性态的植物病原菌。我们根据相关物种的同源序列设计了简并引物，以扩增芹菜尾孢菌、芹菜生尾孢菌、甜菜尾孢菌、玉米尾孢菌和玉蜀黍尾孢菌交配型基因的部分序列。采用染色体步移法来确定这些物种交配型基因的全长。设计引物以扩增并测序其他物种交配型基因的同源部分。对这些序列的系统发育分析表明，芹菜尾孢菌复合种的成员间差异很小，而玉米尾孢菌和玉蜀黍尾孢菌则有所不同。与芹菜尾孢菌（MAT1）和芹菜生尾孢菌（MAT2）的单一交配型相反，甜菜尾孢菌、玉米尾孢菌和玉蜀黍尾孢菌群体中两种交配型的比例大致相等，这表明这些物种中的一些可能存在有性生殖周期。
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