Ellmark Peter, Ingvarsson Johan, Carlsson Anders, Lundin B Samuel, Wingren Christer, Borrebaeck Carl A K
Department of Immunotechnology, Lund University, BMC D13, SE-22184 Lund, Sweden.
Mol Cell Proteomics. 2006 Sep;5(9):1638-46. doi: 10.1074/mcp.M600170-MCP200. Epub 2006 Jul 13.
Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments (SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and -negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these complex tissue proteomes, as well as biomarkers such as IL-9, IL-11, and MCP-4, previously not found in these diseases. In a longer perspective, this study may improve the understanding of H. pylori-induced stomach cancer and lead to development of improved diagnostics.
基于抗体微阵列的技术是蛋白质组学、靶点发现和差异分析中一种强大的新兴工具。在此，我们报告了第一项使用重组抗体片段构建大规模抗体微阵列的研究，该阵列由127种针对大多数免疫调节抗原的不同抗体组成。这些阵列基于设计用于实现高芯片稳定性和功能的单框架重组抗体片段（SinFabs），并用于分析幽门螺杆菌阳性和阴性患者的恶性和正常胃组织样本。我们的结果表明，从这些复杂的组织蛋白质组中可以识别出与肿瘤以及感染相关的独特蛋白质表达特征，以及白细胞介素-9、白细胞介素-11和单核细胞趋化蛋白-4等此前在这些疾病中未发现的生物标志物。从长远来看，这项研究可能会增进对幽门螺杆菌诱发胃癌的理解，并推动改进诊断方法的开发。
