• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

预后生物标志物MCP-4通过p38丝裂原活化蛋白激酶途径触发卵巢癌上皮-间质转化。

Prognostic biomarker MCP-4 triggers epithelial-mesenchymal transition the p38 MAPK pathway in ovarian cancer.

作者信息

Li Siting, Hu Yuexin, Liu Ouxuan, Li Xiao, Lin Bei

机构信息

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Key Laboratory of Maternal-Fetal Medicine of Liaoning Province, Key Laboratory of Obstetrics and Gynecology of Higher Education of Liaoning Province, Shenyang, China.

出版信息

Front Oncol. 2022 Dec 1;12:1034737. doi: 10.3389/fonc.2022.1034737. eCollection 2022.

DOI:10.3389/fonc.2022.1034737
PMID:36531002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9751588/
Abstract

BACKGROUND

Monocyte chemoattractant protein-4 (MCP-4/CCL13) is a proinflammatory factor that is overexpressed in various malignant tumors and may play an important role in tumor progression and metastasis. However, its role and mechanism of action in ovarian cancer remains unknown.

METHODS

Immunohistochemistry (IHC) was performed to detect the expression of MCP-4 in ovarian cancer tissues, and the effect of MCP-4 on patient survival and prognosis was analyzed. Overexpression and suppression of MCP-4 in ovarian cancer cell lines were then established, and their effects on cell invasion, migration, and apoptosis were studied. ES-2 cell lines were employed to establish a peritoneal dissemination model in nude mice. Western blotting was performed to detect the expression of epithelial mesenchymal transition (EMT) markers and the p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway.

RESULTS

MCP-4 was highly expressed in ovarian cancer tissues and its expression level was related to the prognosis of patients with ovarian cancer. MCP-4 overexpression promoted the migration and invasion of ovarian cancer cells but inhibited apoptosis. MCP-4 overexpression increased the expression of MMP-2, MMP-9, N-cadherin, vimentin and Bcl2/Bax and decreased the expression of E-cadherin. MCP-4 overexpression increased the phosphorylation of the p38 MAPK pathway. The inhibition of MCP-4 expression indicated an opposite trend. experiments have also confirmed that MCP-4 overexpression can promote metastasis of ovarian cancer.

CONCLUSION

MCP-4 promotes ovarian cancer progression through the p38 MAPK signaling pathway, and may be a potential biomarker and therapeutic target for ovarian cancer.

摘要

背景

单核细胞趋化蛋白-4（MCP-4/CCL13）是一种促炎因子，在多种恶性肿瘤中过表达，可能在肿瘤进展和转移中起重要作用。然而，其在卵巢癌中的作用及作用机制尚不清楚。

方法

采用免疫组织化学（IHC）检测MCP-4在卵巢癌组织中的表达，并分析MCP-4对患者生存和预后的影响。随后在卵巢癌细胞系中建立MCP-4的过表达和抑制模型，研究其对细胞侵袭、迁移和凋亡的影响。利用ES-2细胞系建立裸鼠腹膜播散模型。采用蛋白质印迹法检测上皮间质转化（EMT）标志物和p38丝裂原活化蛋白激酶（p38 MAPK）信号通路的表达。

结果

MCP-4在卵巢癌组织中高表达，其表达水平与卵巢癌患者的预后相关。MCP-4过表达促进卵巢癌细胞的迁移和侵袭，但抑制凋亡。MCP-4过表达增加MMP-2、MMP-9、N-钙黏蛋白、波形蛋白和Bcl2/Bax的表达，降低E-钙黏蛋白的表达。MCP-4过表达增加p38 MAPK通路的磷酸化。抑制MCP-4表达则呈现相反趋势。实验还证实MCP-4过表达可促进卵巢癌转移。

结论

MCP-4通过p38 MAPK信号通路促进卵巢癌进展，可能是卵巢癌潜在的生物标志物和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/efcbcf6adf44/fonc-12-1034737-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/8e7ff1ada3b8/fonc-12-1034737-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/dfd6d4491226/fonc-12-1034737-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/bbd94dd50a5d/fonc-12-1034737-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/af7c280e3fc3/fonc-12-1034737-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/ca898fd9eb57/fonc-12-1034737-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/efcbcf6adf44/fonc-12-1034737-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/8e7ff1ada3b8/fonc-12-1034737-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/dfd6d4491226/fonc-12-1034737-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/bbd94dd50a5d/fonc-12-1034737-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/af7c280e3fc3/fonc-12-1034737-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/ca898fd9eb57/fonc-12-1034737-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16e2/9751588/efcbcf6adf44/fonc-12-1034737-g006.jpg

相似文献

1
Prognostic biomarker MCP-4 triggers epithelial-mesenchymal transition the p38 MAPK pathway in ovarian cancer.预后生物标志物MCP-4通过p38丝裂原活化蛋白激酶途径触发卵巢癌上皮-间质转化。
Front Oncol. 2022 Dec 1;12:1034737. doi: 10.3389/fonc.2022.1034737. eCollection 2022.
2
Galectin-1 induces metastasis and epithelial-mesenchymal transition (EMT) in human ovarian cancer cells via activation of the MAPK JNK/p38 signalling pathway.半乳糖凝集素-1通过激活丝裂原活化蛋白激酶JNK/p38信号通路诱导人卵巢癌细胞发生转移和上皮-间质转化（EMT）。
Am J Transl Res. 2019 Jun 15;11(6):3862-3878. eCollection 2019.
3
Human chorionic gonadotropin β regulates epithelial-mesenchymal transition and metastasis in human ovarian cancer.人绒毛膜促性腺激素β调节人卵巢癌中的上皮-间质转化和转移。
Oncol Rep. 2017 Sep;38(3):1464-1472. doi: 10.3892/or.2017.5818. Epub 2017 Jul 14.
4
ROR2 promotes the epithelial-mesenchymal transition by regulating MAPK/p38 signaling pathway in breast cancer.ROR2 通过调节 MAPK/p38 信号通路促进乳腺癌的上皮-间质转化。
J Cell Biochem. 2020 Oct;121(10):4142-4153. doi: 10.1002/jcb.29666. Epub 2020 Feb 12.
5
Effects of Platycodon grandiflorus on doxorubicin resistance and epithelial-mesenchymal transition of breast cancer cells via the p38 mitogen-activated protein kinase pathway.桔梗对乳腺癌细胞多柔比星耐药及上皮-间质转化的影响及其作用机制研究。
J Mol Histol. 2024 Dec;55(6):1307-1314. doi: 10.1007/s10735-024-10271-9. Epub 2024 Sep 24.
6
Lobeglitazone, A Peroxisome Proliferator-Activated Receptor-Gamma Agonist, Inhibits Papillary Thyroid Cancer Cell Migration and Invasion by Suppressing p38 MAPK Signaling Pathway.罗格列酮，一种过氧化物酶体增殖物激活受体-γ激动剂，通过抑制 p38 MAPK 信号通路抑制甲状腺乳头状癌细胞迁移和侵袭。
Endocrinol Metab (Seoul). 2021 Oct;36(5):1095-1110. doi: 10.3803/EnM.2021.1155. Epub 2021 Oct 14.
7
HPIP promotes epithelial-mesenchymal transition and cisplatin resistance in ovarian cancer cells through PI3K/AKT pathway activation.HPIP通过激活PI3K/AKT信号通路促进卵巢癌细胞上皮-间质转化和顺铂耐药。
Cell Oncol (Dordr). 2017 Apr;40(2):133-144. doi: 10.1007/s13402-016-0308-2. Epub 2016 Dec 30.
8
Downregulation of SEMA4C Inhibit Epithelial-Mesenchymal Transition (EMT) and the Invasion and Metastasis of Cervical Cancer Cells via Inhibiting Transforming Growth Factor-beta 1 (TGF-β1)-Induced Hela cells p38 Mitogen-Activated Protein Kinase (MAPK) Activation.下调 SEMA4C 通过抑制转化生长因子-β1（TGF-β1）诱导的 Hela 细胞 p38 丝裂原活化蛋白激酶（MAPK）激活抑制宫颈癌上皮间质转化（EMT）和侵袭转移。
Med Sci Monit. 2020 Jan 17;26:e918123. doi: 10.12659/MSM.918123.
9
The involvement of FBP1 in prostate cancer cell epithelial mesenchymal transition, invasion and metastasis by regulating the MAPK signaling pathway.FBP1 通过调节 MAPK 信号通路参与前列腺癌细胞上皮间质转化、侵袭和转移。
Cell Cycle. 2019 Oct;18(19):2432-2446. doi: 10.1080/15384101.2019.1648956. Epub 2019 Aug 25.
10
SCAMP3 is regulated by miR-128-3p and promotes the metastasis of hepatocellular carcinoma cells through EGFR-MAPK p38 signaling pathway.SCAMP3受miR-128-3p调控，并通过EGFR-MAPK p38信号通路促进肝癌细胞的转移。
Am J Transl Res. 2020 Dec 15;12(12):7870-7884. eCollection 2020.

引用本文的文献

1
Amlodipine inhibits Synaptotagmin-4's oncogenic activity on gastric cancer proliferation by targeting calcium signaling.氨氯地平通过靶向钙信号抑制突触结合蛋白-4 对胃癌增殖的致癌活性。
Funct Integr Genomics. 2024 Apr 18;24(3):77. doi: 10.1007/s10142-024-01345-8.
2
Macrophages reprogramming driven by cancer-associated fibroblasts under FOLFIRINOX treatment correlates with shorter survival in pancreatic cancer.在 FOLFIRINOX 治疗下，癌症相关成纤维细胞驱动的巨噬细胞重编程与胰腺癌患者的生存时间更短相关。
Cell Commun Signal. 2024 Jan 2;22(1):1. doi: 10.1186/s12964-023-01388-7.
3
CCL13 and human diseases.

本文引用的文献

1
Functional Roles of JNK and p38 MAPK Signaling in Nasopharyngeal Carcinoma.JNK 和 p38 MAPK 信号通路在鼻咽癌中的功能作用。
Int J Mol Sci. 2022 Jan 20;23(3):1108. doi: 10.3390/ijms23031108.
2
Emerging targeted drug delivery strategies toward ovarian cancer.新兴的卵巢癌靶向药物递送策略。
Adv Drug Deliv Rev. 2021 Nov;178:113969. doi: 10.1016/j.addr.2021.113969. Epub 2021 Sep 9.
3
Chemokine signaling in cancer-stroma communications.趋化因子在癌症-基质通讯中的信号传导
CCL13 与人类疾病。
Front Immunol. 2023 Apr 19;14:1176639. doi: 10.3389/fimmu.2023.1176639. eCollection 2023.
J Cell Commun Signal. 2021 Sep;15(3):361-381. doi: 10.1007/s12079-021-00621-7. Epub 2021 Jun 4.
4
Immune and barrier characterization of atopic dermatitis skin phenotype in Tanzanian patients.坦桑尼亚患者特应性皮炎皮肤表型的免疫和屏障特征。
Ann Allergy Asthma Immunol. 2021 Sep;127(3):334-341. doi: 10.1016/j.anai.2021.04.023. Epub 2021 May 9.
5
Chemokines and the immune response to cancer.趋化因子与癌症的免疫反应。
Immunity. 2021 May 11;54(5):859-874. doi: 10.1016/j.immuni.2021.01.012. Epub 2021 Apr 10.
6
The Roles of Stroma-Derived Chemokine in Different Stages of Cancer Metastases.基质衍生趋化因子在癌症转移不同阶段的作用。
Front Immunol. 2020 Dec 22;11:598532. doi: 10.3389/fimmu.2020.598532. eCollection 2020.
7
Monocyte Chemoattractant Protein-1 promotes cancer cell migration via c-Raf/MAPK/AP-1 pathway and MMP-9 production in osteosarcoma.单核细胞趋化蛋白-1 通过 c-Raf/MAPK/AP-1 通路和 MMP-9 产生促进骨肉瘤中癌细胞迁移。
J Exp Clin Cancer Res. 2020 Nov 23;39(1):254. doi: 10.1186/s13046-020-01756-y.
8
CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of the Ligands of Receptors CCR1, CCR2, CCR3, and CCR4.CC 趋化因子在肿瘤中的作用：配体 CCR1、CCR2、CCR3 和 CCR4 的促癌和抗癌特性综述。
Int J Mol Sci. 2020 Nov 9;21(21):8412. doi: 10.3390/ijms21218412.
9
Treatment of epithelial ovarian cancer.上皮性卵巢癌的治疗。
BMJ. 2020 Nov 9;371:m3773. doi: 10.1136/bmj.m3773.
10
PDK1 promotes ovarian cancer metastasis by modulating tumor-mesothelial adhesion, invasion, and angiogenesis via α5β1 integrin and JNK/IL-8 signaling.丙酮酸脱氢酶激酶1（PDK1）通过α5β1整合素和JNK/IL-8信号通路调节肿瘤-间皮细胞黏附、侵袭和血管生成，从而促进卵巢癌转移。
Oncogenesis. 2020 Feb 18;9(2):24. doi: 10.1038/s41389-020-0209-0.