Sisson P R, Nagy E, Kòczián Z, Veszelovsky E, Freeman R
Public Health Laboratory, General Hospital, Newcastle upon Tyne, UK.
Antonie Van Leeuwenhoek. 1998 May;73(4):327-30. doi: 10.1023/a:1001780407425.
Pyrolysis mass spectrometry was used to characterise Staphylococcus aureus isolates from an outbreak of postoperative wound infections on a mixed surgical ward. The PyMS results were compared with those of phage typing. Both suggested a single strain of S. aureus, of phage type 3C, 55, 71, was responsible for six of the 13 wound infections. PyMS differentiated an isolate from a member of staff of similar phage type to the epidemic strain, which had previously been considered to be the point source for the outbreak. PyMS is a rapid and inexpensive technique for investigating nosocomial outbreaks, including those caused by S. aureus and, in this instance, was more discriminatory than phage typing.
热解质谱法用于对混合外科病房术后伤口感染暴发中分离出的金黄色葡萄球菌菌株进行特征分析。将热解质谱分析结果与噬菌体分型结果进行了比较。两者均表明，噬菌体分型为3C、55、71的单一金黄色葡萄球菌菌株导致了13例伤口感染中的6例。热解质谱法区分出了一名工作人员身上分离出的与流行菌株噬菌体分型相似的菌株，该菌株此前被认为是此次暴发的源头。热解质谱法是一种用于调查医院感染暴发的快速且廉价的技术，包括由金黄色葡萄球菌引起的感染暴发，在这种情况下，它比噬菌体分型更具鉴别力。
