卵胞浆内单精子注射后未受精卵子的直流电激活结果
Results of direct current electrical activation of failed-to-fertilize oocytes after intracytoplasmic sperm injection.
作者信息
Manipalviratn Somjate, Ahnonkitpanit Vichuda, Numchaisrika Pranee, Chompurat Doenthip, Pansatha Jiraporn, Suwajanakorn Somchai
机构信息
Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
J Reprod Med. 2006 Jun;51(6):493-9.
OBJECTIVE
To assess the embryo formation rate after electrical activation on failed-to-fertilize oocytes after intracytoplasmic sperm injection (ICSI).
STUDY DESIGN
A prospective, randomized, experimental study was conducted in our research laboratory at the Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, between January and September 2004. One hundred failed-to-fertilize oocytes after ICSI were randomly assigned by stratified allocation according to oocyte grading before ICSI. Fifty unfertilized oocytes were activated with 3 consecutive 40-/microsec pulses of 1.5 kV/cm direct electrical current in buffer solution. The other 50 unfertilized oocytes, composing the control group, were treated in same way but without electrical activation. The embryo formation rates between the groups were compared.
RESULTS
Embryo formation rates in the electrically activated group were 80% as compared with 16% in the control group (p < 0.001).
CONCLUSION
Failed-to-fertilize oocytes after ICSI seem to be able to resume embryonic development after electrical activation.
目的
评估卵胞浆内单精子注射（ICSI）后未受精的卵母细胞经电激活后的胚胎形成率。
研究设计
2004年1月至9月间，在朱拉隆功大学医学院妇产科生殖医学科的研究实验室进行了一项前瞻性、随机、实验性研究。对ICSI后未受精的100个卵母细胞，根据ICSI前的卵母细胞分级采用分层分配法随机分组。50个未受精的卵母细胞在缓冲溶液中用1.5 kV/cm的直流电连续施加3个40微秒的脉冲进行激活。另外50个未受精的卵母细胞作为对照组，处理方式相同但不进行电激活。比较两组之间的胚胎形成率。
结果
电激活组的胚胎形成率为80%，而对照组为16%（p < 0.001）。
结论
ICSI后未受精的卵母细胞经电激活后似乎能够恢复胚胎发育。
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