Huang Bin, Chu Chien-Hua, Chen Shu-Ling, Juan Hsueh-Fen, Chen Yih-Ming
Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
Cell Mol Biol Lett. 2006;11(2):264-78. doi: 10.2478/s11658-006-0021-7.
Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.
绿豆CYP90A2是一种假定的油菜素内酯（BR）合成基因，与拟南芥CPD基因具有77%的同源性。它受到冷胁迫的强烈抑制。这表明用BR进行外源处理可以使植物从冷胁迫导致的生长抑制中恢复。在本研究中，我们使用蛋白质组学来研究在冷胁迫条件下绿豆上胚轴是否受油菜素内酯调控。最初因冷胁迫生长受到抑制的绿豆上胚轴在用24-表油菜素内酯处理后部分恢复了伸长能力；17种因冷胁迫而下调的蛋白质重新上调。这些上调的蛋白质参与蛋氨酸同化、ATP合成、细胞壁构建和应激反应。这与蛋氨酸合酶和S-腺苷-L-蛋氨酸合成酶的重新上调一致，因为用DL-蛋氨酸进行外源处理可以部分恢复冷胁迫抑制的绿豆上胚轴伸长。这是首次为绿豆物种建立的蛋白质组。研究了油菜素内酯与冷胁迫条件之间的调控关系，并在此讨论了可能的机制。
