黑麦基因组中微卫星标记的鉴定
Identification of microsatellite markers in the rye genome.
作者信息
Bolibok Hanna, Rakoczy-Trojanowska Monika, Wyrzykowska Małgorzata, Radecka Magdalena, Orczyk Wacław
机构信息
Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 166, Warszawa, Poland.
出版信息
Cell Mol Biol Lett. 2006;11(2):291-8. doi: 10.2478/s11658-006-0023-5.
Abstract
The rye genomic library, which consists of DNA fragments in the range of 0.5-1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.
摘要
对由0.5 - 1.1 kb范围内的DNA片段组成的黑麦基因组文库进行筛选，以寻找三核苷酸、四核苷酸和复合微卫星序列。在分析的160万个克隆中，102个克隆呈阳性，41个适合用于SSR引物对设计。26对引物扩增出了特异性产物，其中6对能够在30个不同遗传背景的黑麦材料中检测到多态性。利用一套中国春 - 帝国小麦 - 黑麦附加系，有可能将3个新鉴定的微卫星定位到3R、4R和7R染色体上。
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