Umubyeyi A N, Martin A, Zissis G, Struelens M, Karita E, Portaels F
Mycobacteriology Unit, Institute of Tropical Medecine, Antwerp, Belgium.
Int J Tuberc Lung Dis. 2006 Jul;10(7):808-11.
To evaluate the performance of the colorimetric resazurin microtiter assay (REMA) method for the detection of ofloxacin resistance.
A panel of 120 multidrug-resistant Mycobacterium tuberculosis strains was tested blindly by the REMA method and compared with the results obtained using the BACTEC 460 method.
A very good correlation was observed between the two methods.
The REMA method is simple, rapid and can be an inexpensive alternative procedure for the rapid detection of anti-tuberculosis drug resistance in laboratories with limited resources.
评估比色刃天青微量滴定法(REMA)检测氧氟沙星耐药性的性能。
采用REMA方法对120株耐多药结核分枝杆菌菌株进行盲法检测,并与使用BACTEC 460方法获得的结果进行比较。
观察到两种方法之间具有很好的相关性。
REMA方法简单、快速,对于资源有限的实验室而言,可作为一种廉价的替代方法用于快速检测抗结核药物耐药性。