Martin A, Paasch F, Von Groll A, Fissette K, Almeida P, Varaine F, Portaels F, Palomino J-C
Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium.
Int J Tuberc Lung Dis. 2009 Oct;13(10):1301-4.
In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics.
To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method.
One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM.
The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days.
TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.
在低收入国家，迫切需要经济的方法来检测结核分枝杆菌对抗生素的敏感性。
评估薄层琼脂（TLA）法快速检测结核分枝杆菌临床分离株对利福平（RMP）、氧氟沙星（OFX）和卡那霉素（KM）的耐药性，并与金标准方法比较，确定其敏感性、特异性和阳性检出时间。
研究了147株结核分枝杆菌临床分离株。对于TLA法，使用含有含RMP、OFX和KM的7H11琼脂的四区培养皿。将结果与用于RMP检测的Bactec MGIT960以及用于OFX和KM检测的比例法进行比较。
RMP和OFX的敏感性和特异性均为100%，KM的敏感性和特异性分别为100%和98.7%。使用TLA四区培养皿能够在中位时间10天内快速检测出对三种抗结核药物RMP、OFX和KM的耐药性。
TLA是检测所研究的三种药物耐药性的准确方法。这种更快的方法操作简单，在资源匮乏地区没有更先进技术时提供了一种替代方法。
