一种在果蝇中进行的高效遗传筛选，用于鉴定具有线粒体功能的核编码基因。
An efficient genetic screen in Drosophila to identify nuclear-encoded genes with mitochondrial function.
作者信息
Liao T S Vivian, Call Gerald B, Guptan Preeta, Cespedes Albert, Marshall Jamie, Yackle Kevin, Owusu-Ansah Edward, Mandal Sudip, Fang Q Angela, Goodstein Gelsey L, Kim William, Banerjee Utpal
机构信息
Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
出版信息
Genetics. 2006 Sep;174(1):525-33. doi: 10.1534/genetics.106.061705. Epub 2006 Jul 18.
Abstract
We conducted a screen for glossy-eye flies that fail to incorporate BrdU in the third larval instar eye disc but exhibit normal neuronal differentiation and isolated 23 complementation groups of mutants. These same phenotypes were previously seen in mutants for cytochrome c oxidase subunit Va. We have molecularly characterized six complementation groups and, surprisingly, each encodes a mitochondrial protein. Therefore, we believe our screen to be an efficient method for identifying genes with mitochondrial function.
摘要
我们对在三龄幼虫眼盘中不能掺入BrdU但表现出正常神经元分化的亮眼果蝇进行了筛选，并分离出23个互补群的突变体。这些相同的表型先前在细胞色素c氧化酶亚基Va的突变体中也观察到。我们已经对六个互补群进行了分子特征分析，令人惊讶的是，每个互补群都编码一种线粒体蛋白。因此，我们认为我们的筛选是鉴定具有线粒体功能基因的一种有效方法。
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