Aslan Kadir, Lakowicz Joseph R, Geddes Chris D
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA.
J Phys Chem B. 2005 Apr 7;109(13):6247-51. doi: 10.1021/jp044235z.
A simple and rapid wet-chemical technique for the deposition of silver triangles on conventional glass substrates, which alleviates the need for lithography, has been developed. The technique is based on the seed-mediated cetyltrimethylammonium-bromide-directed growth of silver triangles on glass surfaces, where smaller spherical silver seeds that were attached to the surface were subsequently converted and grown into silver triangles in the presence of a cationic surfactant and silver ions. The size of the silver triangles was controlled by sequential immersion of silver seed-coated glass substrates into a growth solution and by the duration time of immersion. Atomic force microscopy studies revealed that the size of the silver triangles ranged between 100 and 500 nm. Interestingly, these new surfaces are a significant improvement over traditional silver island films for applications in metal-enhanced fluorescence. A routine 16-fold enhancement in emission intensity was typically observed, for protein-immobilized indocyanine green, with a relatively very low loading density of silver triangles on the glass surface.
已经开发出一种简单快速的湿化学技术，用于在传统玻璃基板上沉积银三角，该技术无需光刻。该技术基于种子介导的十六烷基三甲基溴化铵定向生长银三角在玻璃表面，其中附着在表面的较小球形银种子随后在阳离子表面活性剂和银离子存在下转化并生长成银三角。通过将涂有银种子的玻璃基板顺序浸入生长溶液中以及浸入持续时间来控制银三角的尺寸。原子力显微镜研究表明，银三角的尺寸在100至500纳米之间。有趣的是，这些新表面在用于金属增强荧光的应用中比传统银岛膜有显著改进。对于固定有蛋白质的吲哚菁绿，通常观察到发射强度有16倍的常规增强，玻璃表面上银三角的负载密度相对非常低。
