Aslan Kadir, Lakowicz Joseph R, Geddes Chris D
Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, Institute of Fluorescence, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, MD 21201, USA.
Anal Bioanal Chem. 2005 Jun;382(4):926-33. doi: 10.1007/s00216-005-3195-3. Epub 2005 Jun 4.
In this critical and timely review, the effects of anisotropic silver nanostructures on the emission intensity and photostability of a key fluorophore that is frequently used in many biological assays is examined. The silver nanostructures consist of triangular, rod-like, and fractal-like nanoparticles of silver deposited on conventional glass substrates. The close proximity to silver nanostructures results in greater intensity and photostability of the fluorophore than for fluorophores solely deposited on glass substrates. These new anisotropic silver nanostructure-coated surfaces show much more favorable effects than silver island films or silver colloid-coated substrates. Subsequently, the use of metal-enhanced fluorescence (MEF) for biosensing applications is discussed.
在这篇重要且及时的综述中,研究了各向异性银纳米结构对许多生物检测中常用的一种关键荧光团的发射强度和光稳定性的影响。银纳米结构由沉积在传统玻璃基板上的三角形、棒状和类分形银纳米颗粒组成。与仅沉积在玻璃基板上的荧光团相比,靠近银纳米结构会使荧光团具有更高的强度和光稳定性。这些新的各向异性银纳米结构涂层表面比银岛膜或银胶体涂层基板显示出更有利的效果。随后,讨论了金属增强荧光(MEF)在生物传感应用中的使用。