Miles J S, Moss J E, Taylor B A, Burchell B, Wolf C R
Imperial Cancer Research Fund, University Department of Biochemistry, Edinburgh, United Kingdom.
Genomics. 1991 Oct;11(2):309-16. doi: 10.1016/0888-7543(91)90137-4.
Probes for cytochrome P450IVA (P450IVA), alpha- and pi-class glutathione S-transferases (GST), and phenol-metabolizing UDP-glucuronyltransferase (UDPGT-K39) detected restriction fragment length variants (RFLVs) between C57BL/6J and DBA/2J mice. These variants were used to map the P450IVA genes (Cyp4 alpha) to chromosome 4, close to Mtv-13 and Pmv-19, midway between brown (b) and Gpd-1; GST alpha genes were mapped to chromosome 9, with a cross-hybridizing sequence mapping to another chromosome; the GST pi genes were mapped to the distal end of chromosome 1 near Pmv-21; one UDPGT-K39 variant to chromosome 1, between Acrg and Emv-17, and another showed linkage to Odc-10 on an unidentified chromosome. No RFLVs were detected with probes for P450IID, P450 reductase, androsterone-metabolizing UDPGT, GST mu, or microsomal GST.
细胞色素P450IVA（P450IVA）、α-和π-类谷胱甘肽S-转移酶（GST）以及代谢苯酚的尿苷二磷酸葡萄糖醛酸基转移酶（UDPGT-K39）的探针检测到C57BL/6J和DBA/2J小鼠之间的限制性片段长度变异（RFLV）。这些变异被用于将P450IVA基因（Cyp4α）定位到4号染色体，靠近Mtv-13和Pmv-19，在棕色（b）和Gpd-1之间的中间位置；GSTα基因被定位到9号染色体，一个交叉杂交序列定位到另一条染色体；GSTπ基因被定位到1号染色体远端靠近Pmv-21的位置；一个UDPGT-K39变异定位到1号染色体，在Acrg和Emv-17之间，另一个显示与一条未鉴定染色体上的Odc-10连锁。用P450IID、P450还原酶、代谢雄甾酮的UDPGT、GSTμ或微粒体GST的探针未检测到RFLV。
