Schollenberger Margit, Müller H-M, Rüfle Melanie, Terry-Jara Helga, Suchy Sybille, Plank Susanne, Drochner W
Institute of Animal Nutrition, Hohenheim University, Emil-Wolff-Str. 10, D-70599 Stuttgart, Germany.
Int J Food Microbiol. 2007 Jan 25;113(2):142-6. doi: 10.1016/j.ijfoodmicro.2006.06.022. Epub 2006 Jul 18.
A total of 45 samples of soy food including whole beans, roasted soy nuts, flour and flakes, textured soy protein, tofu, proteinisolate including infant formulas and fermented products (soy sauce) were randomly collected in food and health food stores and analysed for Fusarium toxins. A spectrum of 13 trichothecenes of the A-type as well as of the B-type were determined by gas chromatography/mass spectrometry, zearalenone (ZEA), alpha- and beta-zearalenol (alpha- and beta-ZOL) by high performance liquid chromatography (HPLC) with fluorescence and UV-detection. Detection limits ranged between 1 and 19 microg/kg. At least one of the toxins investigated was detected in 11 out of a total of 45 samples of soy food belonging to different commodities. Scirpentriol (SCIRP), 15-monoacetoxyscirpenol, 4,15-diacetoxyscirpenol, T-2 tetraol, HT-2 toxin, deoxynivalenol (DON), 15- and 3-acetyldeoxynivalenol, ZEA, alpha- and beta-ZOL were detected in at least one sample, T-2 triol, T-2, NEO, NIV and FUS-X were not detected in any sample. Five out of 11 samples were positive for one toxin, one sample for two, three, six or seven toxins, two samples for 5 toxins, demonstrating the possibility of a contamination of soy food with a spectrum of Fusarium toxins. SCIRP, DON and ZEA were found up to 108, 260 and 214 microg/kg, the other toxins did not exceed 61 microg/kg. A first insight into the contamination of soy food with a broad spectrum of Fusarium toxins is provided.
总共从食品店和保健食品店随机采集了45份大豆食品样本，包括整粒豆、烤大豆坚果、面粉和薄片、组织化大豆蛋白、豆腐、蛋白分离物（包括婴儿配方奶粉）以及发酵产品（酱油），并对其进行了镰刀菌毒素分析。通过气相色谱/质谱法测定了13种A 型和B型单端孢霉烯族毒素，通过高效液相色谱（HPLC）结合荧光和紫外检测法测定了玉米赤霉烯酮（ZEA）、α-和β-玉米赤霉醇（α-和β-ZOL）。检测限在1至19微克/千克之间。在总共45份属于不同商品的大豆食品样本中，有11份至少检测出了一种所研究的毒素。在至少一个样本中检测到了蛇形菌素（SCIRP）、15-单乙酰氧基蛇形菌素、4,15-二乙酰氧基蛇形菌素、T-2四醇、HT-2毒素、脱氧雪腐镰刀菌烯醇（DON）、15-和3-乙酰脱氧雪腐镰刀菌烯醇、ZEA、α-和β-ZOL，在任何样本中均未检测到T-2三醇、T-2、新茄病镰刀菌烯醇（NEO）、雪腐镰刀菌烯醇（NIV）和FUS-X。11个样本中有5个样本对一种毒素呈阳性，1个样本对两种、三种、六种或七种毒素呈阳性，2个样本对5种毒素呈阳性，这表明大豆食品有可能受到多种镰刀菌毒素的污染。SCIRP、DON和ZEA的含量分别高达108、260和214微克/千克，其他毒素含量不超过61微克/千克。本文首次对大豆食品受到多种镰刀菌毒素污染的情况进行了研究。
