Peskircioglu L, Atac F B, Erdem S R, Deveci S, Verdi H, Ozkardeş H
Department of Urology, Baskent University, School of Medicine, Bahçelievler, Ankara, Turkey.
Int J Impot Res. 2007 Mar-Apr;19(2):149-53. doi: 10.1038/sj.ijir.3901501. Epub 2006 Jul 27.
The objective of the study was to determine the association between intron 4 variable number of tandem repeats (VNTR), E298A and IVF 23+10 G/T polymorphisms of ec-NOS gene and sildenafil responsiveness in patients with erectile dysfunction (ED). Ninety-six patients who were evaluated for ED between November 2003 and June 2004 and 167 healthy individuals representing the normal population as controls were included in the present study. The patients were evaluated by medical history, five-item version of International Index of Erectile Function, serum glucose, testosterone levels and lipid profiles. Sixty-seven patients received four consecutive doses of sildenafil from 25 to 100 mg according to the response. The ec-NOS gene intron 4 VNTR, E298A and IVF 23+10 G/T polymorphisms were evaluated in the isolated DNA blood samples obtained from the patient group with ED (n=96), from the group received sildenafil (n=67) and from the healthy group (n=167). Genotype distributions of ec-NOS gene intron 4, E298A and IVF 23+10 G/T polymorphisms in the patient group were similar to those in the healthy group. The frequency of the ec-NOS gene intron 4 genotype were found as bb=41.7%, ab=50% and aa=8.3% in the sildenafil responders and bb=93.5% and ba=6.5% in the sildenafil non-responders. This finding was statistically significant. Statistical analysis of ec-NOS gene E298A and IVF 23+10 G/T polymorphisms did not reveal any significant difference between sildenafil responders and non-responders. These findings may indicate that 'a' allele of ec-NOS gene intron 4 VNTR polymorphism associates with a better sildenafil response.
本研究的目的是确定内皮型一氧化氮合酶（ec-NOS）基因内含子4可变串联重复序列（VNTR）、E298A和IVF 23+10 G/T多态性与勃起功能障碍（ED）患者西地那非反应性之间的关联。本研究纳入了2003年11月至2004年6月期间接受ED评估的96例患者以及167名代表正常人群的健康个体作为对照。通过病史、国际勃起功能指数五项版本、血糖、睾酮水平和血脂谱对患者进行评估。67例患者根据反应情况连续接受四剂25至100毫克的西地那非治疗。在从ED患者组（n=96）、接受西地那非治疗的组（n=67）和健康组（n=167）获取的分离DNA血样中评估ec-NOS基因内含子4 VNTR、E298A和IVF 23+10 G/T多态性。患者组中ec-NOS基因内含子4、E298A和IVF 23+10 G/T多态性的基因型分布与健康组相似。在西地那非反应者中，ec-NOS基因内含子4基因型的频率为bb=41.7%、ab=50%、aa=8.3%；在西地那非无反应者中，bb=93.5%、ba=6.5%。这一发现具有统计学意义。ec-NOS基因E298A和IVF 23+10 G/T多态性的统计分析未显示西地那非反应者和无反应者之间有任何显著差异。这些发现可能表明ec-NOS基因内含子4 VNTR多态性的“a”等位基因与更好的西地那非反应相关。
