Wissmann Gustavo, Alvarez-Martinez Míriam J, Meshnick Steven R, Dihel Ada R S, Prolla João C
Hospital de Clínicas de Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
J Eukaryot Microbiol. 2006 Jul-Aug;53(4):305-7. doi: 10.1111/j.1550-7408.2006.00104.x.
Several studies from developed countries have documented the association between trimethoprim-sulfamethoxazole prophylaxis failure and mutations in the Pneumocystis jirovecii gene coding for dihydropteroate synthase (DHPS). DNA was extracted from Giemsa-stained smears of 70 patients with P. jirovecii pneumonia seen in Porto Alegre, Brazil, from 1997 to 2004. Successful PCR amplification of the DHPS locus was obtained in 57 of 70 cases (81.4%), including five cases (8.7%) that had used sulfa prophylaxis. No DHPS gene mutations were seen. These results suggest that DHPS mutations are currently as rare in Brazil as in other developing countries.
来自发达国家的多项研究记录了甲氧苄啶-磺胺甲恶唑预防失败与编码二氢蝶酸合酶（DHPS）的耶氏肺孢子菌基因中的突变之间的关联。从1997年至2004年在巴西阿雷格里港就诊的70例耶氏肺孢子菌肺炎患者的吉姆萨染色涂片提取DNA。70例中的57例（81.4%）成功进行了DHPS基因座的PCR扩增，其中包括5例（8.7%）曾使用磺胺类药物预防的病例。未发现DHPS基因突变。这些结果表明，目前DHPS突变在巴西与其他发展中国家一样罕见。
