通过对个体牛奶样本进行两种抗体检测法检测希腊奶牛群中的犬新孢子虫感染情况。
Neospora caninum infection in Greek dairy cattle herds detected by two antibody assays in individual milk samples.
作者信息
Sotiraki S, Brozos C, Samartzi F, Schares G, Kiossis E, Conraths F J
机构信息
Veterinary Research Institute, National Agricultural Research Foundation, Ionia, 57008 Thessaloniki, Greece.
出版信息
Vet Parasitol. 2008 Mar 25;152(1-2):79-84. doi: 10.1016/j.vetpar.2007.11.016. Epub 2007 Nov 21.
A survey to demonstrate the presence and the extent of Neospora caninum infection in dairy cattle was carried out in Greece. Seven hundred and seventy-seven (777) individual milk samples from all milking cows in 10 dairy herds were tested using an ELISA (p38-milk-ELISA) and immunoblot analysis. The herd prevalence was 80.0% and 100.0%, while the overall prevalence was 15.2% (ranging between 0.0% and 38.9%) and 27.9% (ranging between 3.4% and 61.6%) based on the results of the ELISA and the immunoblot test, respectively. In conclusion, N. caninum infection is present among dairy cattle in Greece, deserving more studies to determine its contribution to abortion problems in Greek dairy herds.
在希腊开展了一项调查，以证明奶牛新孢子虫感染的存在情况和感染程度。使用酶联免疫吸附测定法（p38-牛奶-酶联免疫吸附测定法）和免疫印迹分析，对来自10个奶牛场所有泌乳奶牛的777份个体牛奶样本进行了检测。基于酶联免疫吸附测定法和免疫印迹试验的结果，牛群患病率分别为80.0%和100.0%，而总体患病率分别为15.2%（范围在0.0%至38.9%之间）和27.9%（范围在3.4%至61.6%之间）。总之，希腊奶牛中存在新孢子虫感染，值得开展更多研究以确定其对希腊奶牛场流产问题的影响。
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