转录因子IIB在体内启动子处的组装需要与RNA聚合酶II接触。
Assembly of transcription factor IIB at a promoter in vivo requires contact with RNA polymerase II.
作者信息
Elsby Laura M, O'Donnell Amanda J M, Green Laura M, Sharrocks Andrew D, Roberts Stefan G E
机构信息
Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
出版信息
EMBO Rep. 2006 Sep;7(9):898-903. doi: 10.1038/sj.embor.7400767. Epub 2006 Jul 28.
Abstract
The general transcription factor TFIIB has a central role in the assembly of the preinitiation complex at the promoter, providing a platform for the entry of RNA polymerase II/TFIIF. We used an RNA interference (RNAi)-based system in which TFIIB expression is ablated in vivo and replaced with a TFIIB derivative that contains a silent mutation and is refractory to the RNAi. Using this approach, we found that transcriptionally defective TFIIB amino-terminal mutants showed distinct effects on the basis of their ability to compete with wild-type TFIIB in vivo. Moreover, analysis of the TFIIB mutant derivatives by chromatin immunoprecipitation showed that promoter occupancy by TFIIB is dependent on the association with RNA polymerase II. Together, our results support a mode of preinitiation complex assembly in which TFIIB/RNA polymerase II recruitment to the promoter occurs in vivo.
摘要
通用转录因子TFIIB在启动子处的起始前复合物组装中起核心作用，为RNA聚合酶II/TFIIF的进入提供了一个平台。我们使用了一种基于RNA干扰（RNAi）的系统，其中TFIIB的表达在体内被消除，并用一种含有沉默突变且对RNAi有抗性的TFIIB衍生物取代。使用这种方法，我们发现转录缺陷的TFIIB氨基末端突变体根据其在体内与野生型TFIIB竞争的能力表现出不同的影响。此外，通过染色质免疫沉淀对TFIIB突变衍生物的分析表明，TFIIB在启动子上的占据取决于与RNA聚合酶II的结合。总之，我们的结果支持一种起始前复合物组装模式，即TFIIB/RNA聚合酶II在体内被招募到启动子上。
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本文引用的文献
1
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Genes Dev. 2005 Oct 15;19(20):2418-23. doi: 10.1101/gad.342405.
2
Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: a model for the structure of the PIC.绘制TFIIB在RNA聚合酶II转录起始前复合物中的位置：转录起始前复合物结构模型
Cell. 2004 Oct 15;119(2):169-80. doi: 10.1016/j.cell.2004.09.028.
3
Structure and mechanism of the RNA polymerase II transcription machinery.RNA聚合酶II转录机制的结构与机理
Nat Struct Mol Biol. 2004 May;11(5):394-403. doi: 10.1038/nsmb763.
4
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Nucleic Acids Res. 2004 Mar 22;32(5):1829-35. doi: 10.1093/nar/gkh504. Print 2004.
5
A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment.TFIIB锌带结构域的一个非保守表面在RNA聚合酶II招募中起直接作用。
Mol Cell Biol. 2004 Apr;24(7):2863-74. doi: 10.1128/MCB.24.7.2863-2874.2004.
6
Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.转录的结构基础：4.5埃分辨率下的RNA聚合酶II-TFIIB共晶体结构
Science. 2004 Feb 13;303(5660):983-8. doi: 10.1126/science.1090838.
7
Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex.TFIIB与RNA聚合酶II的结合：绘制起始前复合物中TFIIB锌带结构域的结合位点。
Mol Cell. 2003 Aug;12(2):437-47. doi: 10.1016/s1097-2765(03)00306-x.
8
Dynamics of enhancer-promoter communication during differentiation-induced gene activation.分化诱导基因激活过程中增强子-启动子通讯的动力学
Mol Cell. 2002 Dec;10(6):1467-77. doi: 10.1016/s1097-2765(02)00786-4.
9
The RNA polymerase II core promoter: a key component in the regulation of gene expression.RNA聚合酶II核心启动子：基因表达调控中的关键组分。
Genes Dev. 2002 Oct 15;16(20):2583-92. doi: 10.1101/gad.1026202.
10
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Mol Cell Biol. 2002 Oct;22(19):6697-705. doi: 10.1128/MCB.22.19.6697-6705.2002.
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