Mohd Masri S, Nazni W A, Lee H L, T Rogayah T A R, Subramaniam S
Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
Trop Biomed. 2005 Dec;22(2):185-9.
Three new techniques of sterilising maggots of Lucilia cuprina for the purpose of debriding intractable wounds were studied. These techniques were utilisation of ultra-violet C (UVC) and maggot sterilisation with disinfectants. The status of sterility was checked on nutrient agar and blood agar and confirmed with staining. The indicators for the effectiveness of the methods were sterility and survival rate of the eggs or larvae. Egg sterilisation with UVC had the lowest hatching rate (16+/-0.00%) while egg sterilisation with disinfectants showed high hatching rate (36.67+/-4.41%) but low maggot survival rate (31.67+/-1.67%). Sterilisation of the maggots was the most suitable, since the survival rate was the highest (88.67+/-0.88%). Complete sterility was achieved in all cases, except that Proteus mirabilis was consistently found. However, the presence of this microorganism was considered beneficial.
研究了三种用于处理嗜尸性绿蝇蛆以清创难愈合伤口的新灭菌技术。这些技术包括使用紫外线C（UVC）和用消毒剂对蛆进行灭菌。在营养琼脂和血琼脂上检查无菌状态，并通过染色进行确认。这些方法有效性的指标是卵或幼虫的无菌状态和存活率。用UVC对卵进行灭菌的孵化率最低（16±0.00%），而用消毒剂对卵进行灭菌的孵化率较高（36.67±4.41%），但蛆的存活率较低（31.67±1.67%）。对蛆进行灭菌是最合适的，因为其存活率最高（88.67±0.88%）。除了始终能检测到奇异变形杆菌外，所有情况下均实现了完全无菌。然而，这种微生物的存在被认为是有益的。
