胰岛素对脂肪组织中丙酮酸脱氢酶的激活作用。胰岛素对丙酮酸脱氢酶磷酸磷酸酶作用的证据。
Activation of pyruvate dehydrogenase in adipose tissue by insulin. Evidence for an effect of insulin on pyruvate dehydrogenase phosphate phosphatase.
作者信息
Mukherjee C, Jungas R L
出版信息
Biochem J. 1975 May;148(2):229-35. doi: 10.1042/bj1480229.
Abstract
- The mechanism by which insulin activates pyruvate dehydrogenase in rat epididymal adipose tissue was further investigated. 2. When crude extracts, prepared from tissue segments previously exposed to insulin (2m-i.u/ml) for 2min, were supplemented with Mg-2+, Ca-2+, glucose and hexokinase and incubated at 30 degrees C, they displayed an enhanced rate of increase in pyruvate dehydrogenase activity compared with control extracts. 3. When similar extracts were instead supplemented with fluoride, ADP, creatine phosphate and creatine kinase, the rate of decrease in pyruvate dehydrogenase activity observed during incubation at 30 degrees C was unaffected by insulin treatment. 4. It is suggested that insulin increases the fraction of pyruvate dehydrogenase present in the tissue in the active dephospho form by increasing the activity of pyruvate dehydrogenase phosphate phosphatase.
摘要
- 进一步研究了胰岛素激活大鼠附睾脂肪组织中丙酮酸脱氢酶的机制。2. 当用先前暴露于胰岛素（2m - i.u/ml）2分钟的组织片段制备的粗提物，补充Mg - 2 +、Ca - 2 +、葡萄糖和己糖激酶，并在30℃孵育时，与对照提取物相比，它们显示出丙酮酸脱氢酶活性增强的增加速率。3. 当类似的提取物改为补充氟化物、ADP、磷酸肌酸和肌酸激酶时，在30℃孵育期间观察到的丙酮酸脱氢酶活性下降速率不受胰岛素处理的影响。4. 提示胰岛素通过增加丙酮酸脱氢酶磷酸酶的活性，增加组织中以活性去磷酸形式存在的丙酮酸脱氢酶的比例。
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