Otsuka Hidenori, Ikeya Takeshi, Okano Teruo, Kataoka Kazunori
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Eur Cell Mater. 2006 Aug 3;12:36-43; discussion 36-43. doi: 10.22203/ecm.v012a04.
This study demonstrates that boronic acid-containing polymers coated onto solid support function as synthetic mitogens for mouse lymphocytes. The polymer was synthesized by radical copolymerization of 3-acrylamidophenylboronic acid with dimethylacrylamide (poly(AAPBA-DMAA)). The boronic acid in the trigonal form in the copolymer activated lymphocytes, probably by crosslinkage to glycoprotein moieties on the plasma membrane surface, as in the case of lectin stimulation. A higher concentration of phenylboronic acid on the copolymer surface resulted in greater activation of lymphocytes, suggesting that the number of phenylboronic acid residues per unit area may be a crucial factor in lymphocyte proliferation. The proliferative response of lymphocytes was also affected by the surface wettability, probably due to a difference in the flexibility of polymer strands at the cell-polymer interface.
本研究表明，涂覆在固体支持物上的含硼酸聚合物可作为小鼠淋巴细胞的合成促细胞分裂剂。该聚合物通过3-丙烯酰胺基苯硼酸与二甲基丙烯酰胺的自由基共聚反应合成（聚（AAPBA-DMAA））。共聚物中三角形式的硼酸激活淋巴细胞，可能是通过与质膜表面糖蛋白部分交联，就像凝集素刺激的情况一样。共聚物表面较高浓度的苯硼酸导致淋巴细胞的更大激活，这表明单位面积苯硼酸残基的数量可能是淋巴细胞增殖的关键因素。淋巴细胞的增殖反应也受表面润湿性的影响，这可能是由于细胞-聚合物界面处聚合物链的柔韧性不同所致。
