Shon Yun-Hee, Park Sun-Dong, Nam Kyung-Soo
Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
J Biochem Mol Biol. 2006 Jul 31;39(4):448-51. doi: 10.5483/bmbrep.2006.39.4.448.
Chemopreventive and cytotoxic effect of genistein against human breast cancer cell lines was investigated. Genistein inhibited cell proliferation in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. Cytochrome P450 (CYP) 1A1-mediated ethoxyresorufin O-deethylase (EROD)activity was inhibited by genistein in a concentration-dependent manner. Genistein significantly inhibited 12-Otetradecanoylphorbol-13-acetate (TPA)-induced cyclooxygenase-2 activity and protein expression at the concentrations of 10 (p < 0.05), 25 (p < 0.05) and 50 mM (p < 0.01). In addition, ornithine decarboxylase (ODC) activity was reduced to 53.8 % of the control after 6 h treatment with 50 mM genistein in MCF-7 breast cancer cells. These results suggest that genistein could be of therapeutic value in preventing human breast cancer.
研究了染料木黄酮对人乳腺癌细胞系的化学预防和细胞毒性作用。染料木黄酮抑制雌激素受体阳性（MCF - 7）和雌激素受体阴性（MDA - MB - 231）人乳腺癌细胞系的细胞增殖。细胞色素P450（CYP）1A1介导的乙氧异吩恶唑酮O - 脱乙基酶（EROD）活性被染料木黄酮以浓度依赖的方式抑制。在浓度为10（p < 0.05）、25（p < 0.05）和50 mM（p < 0.01）时，染料木黄酮显著抑制12 - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的环氧合酶 - 2活性和蛋白表达。此外，在MCF - 7乳腺癌细胞中用50 mM染料木黄酮处理6小时后，鸟氨酸脱羧酶（ODC）活性降至对照的53.8%。这些结果表明染料木黄酮在预防人类乳腺癌方面可能具有治疗价值。
