Branda John A, Kratz Alexander
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Am J Clin Pathol. 2006 Aug;126(2):248-54. doi: 10.1309/1G5H-B7PA-K8V0-KAR7.
We studied the effects of yeast in peripheral blood samples on results reported by an ADVIA 120/2120 Hematology System (Bayer HealthCare, Diagnostics Division, Tarrytown, NY). In a simulated candidemia model, very high concentrations (1-5 x 10(8) colony-forming units [CFU]/mL) of Candida glabrata and Candida parapsilosis caused a spuriously elevated platelet count. No such effect was observed with Candida albicans. All 3 yeast species, when present at a concentration of 1-5 x 10(6) CFU/mL or greater, increased the automated WBC counts significantly and in a dose-dependent manner. The yeast cells were mainly misidentified as lymphocytes. All spurious results were flagged by the cell counter for microscopic review. We conclude that although the presence of yeast in a blood sample can interfere with the ADVIA 120/2120 Hematology System, compromised results are appropriately flagged by the instrument and are seen only when the yeast concentration is very high.
我们研究了外周血样本中的酵母对ADVIA 120/2120血液学系统（拜耳医疗保健公司诊断部，纽约州塔里敦）所报告结果的影响。在一个模拟念珠菌血症模型中，非常高浓度（1 - 5×10⁸ 菌落形成单位[CFU]/mL）的光滑念珠菌和近平滑念珠菌导致血小板计数假性升高。白色念珠菌未观察到这种效应。当所有3种酵母浓度为1 - 5×10⁶ CFU/mL或更高时，会以剂量依赖方式显著增加白细胞自动计数。酵母细胞主要被误识别为淋巴细胞。所有假性结果均被细胞计数仪标记以供显微镜检查。我们得出结论，尽管血液样本中酵母的存在会干扰ADVIA 120/2120血液学系统，但仪器会对受损结果进行适当标记，且只有当酵母浓度非常高时才会出现这种情况。
