• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

过量表达真菌来源几丁质酶的转基因烟草植株对生物和非生物胁迫因子表现出增强的抗性。

Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents.

作者信息

de las Mercedes Dana María, Pintor-Toro José A, Cubero Beatriz

机构信息

Instituto de Recursos Naturales y Agrobiología de Sevilla, Consejo Superior de Investigaciones Científicas, 41012-Seville, Spain.

出版信息

Plant Physiol. 2006 Oct;142(2):722-30. doi: 10.1104/pp.106.086140. Epub 2006 Aug 4.

DOI:10.1104/pp.106.086140
PMID:16891545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1586035/
Abstract

Genes encoding defense-related proteins have been used to alter the resistance of plants to pathogens and other environmental challenges, but no single fungal gene overexpression has produced broad-spectrum stress resistance in transgenic lines. We have generated transgenic tobacco (Nicotiana tabacum) lines that overexpress the endochitinases CHIT33 and CHIT42 from the mycoparasitic fungus Trichoderma harzianum and have evaluated their tolerance to biotic and abiotic stress. Both CHIT33 and CHIT42, individually, conferred broad resistance to fungal and bacterial pathogens, salinity, and heavy metals. Such broad-range protective effects came off with no obvious detrimental effect on the growth of tobacco plants.

摘要

编码防御相关蛋白的基因已被用于改变植物对病原体和其他环境挑战的抗性，但在转基因品系中，单一真菌基因的过表达并未产生广谱的胁迫抗性。我们构建了过表达来自哈茨木霉的内切几丁质酶CHIT33和CHIT42的转基因烟草（烟草）品系，并评估了它们对生物和非生物胁迫的耐受性。单独的CHIT33和CHIT42均赋予了对真菌和细菌病原体、盐度和重金属的广泛抗性。这种广泛的保护作用并未对烟草植株的生长产生明显的不利影响。

相似文献

1
Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents.过量表达真菌来源几丁质酶的转基因烟草植株对生物和非生物胁迫因子表现出增强的抗性。
Plant Physiol. 2006 Oct;142(2):722-30. doi: 10.1104/pp.106.086140. Epub 2006 Aug 4.
2
Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain.通过添加纤维素结合结构域提高哈茨木霉CECT 2413的抗真菌和几丁质酶比活性。
Appl Microbiol Biotechnol. 2004 Jun;64(5):675-85. doi: 10.1007/s00253-003-1538-6. Epub 2004 Jan 23.
3
The LysM receptor-like kinase LysM RLK1 is required to activate defense and abiotic-stress responses induced by overexpression of fungal chitinases in Arabidopsis plants.拟南芥中 LysM 受体样激酶 LysM RLK1 对于真菌几丁质酶过表达诱导的防御和非生物胁迫反应的激活是必需的。
Mol Plant. 2012 Sep;5(5):1113-24. doi: 10.1093/mp/sss021. Epub 2012 Mar 28.
4
Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum.哈茨木霉中几丁质酶33（chit33）基因表达的调控
Curr Genet. 2001 Jan;38(6):335-42. doi: 10.1007/s002940000169.
5
Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride.通过用来自深绿木霉的细胞壁降解酶基因转化来增强水稻对真菌病原体的抗性。
J Zhejiang Univ Sci. 2004 Feb;5(2):133-6. doi: 10.1007/BF02840913.
6
Addition of substrate-binding domains increases substrate-binding capacity and specific activity of a chitinase from Trichoderma harzianum.添加底物结合结构域可提高哈茨木霉几丁质酶的底物结合能力和比活性。
FEMS Microbiol Lett. 2001 Apr 20;198(1):57-63. doi: 10.1111/j.1574-6968.2001.tb10619.x.
7
Transplastomic Nicotiana benthamiana plants expressing multiple defence genes encoding protease inhibitors and chitinase display broad-spectrum resistance against insects, pathogens and abiotic stresses.转叶绿体拟南芥植物表达多种防御基因，编码蛋白酶抑制剂和几丁质酶，表现出对昆虫、病原体和非生物胁迫的广谱抗性。
Plant Biotechnol J. 2014 May;12(4):503-15. doi: 10.1111/pbi.12157. Epub 2014 Jan 30.
8
Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea.构建哈茨木霉 P1 几丁质 42 基因工程链霉菌 A01 转化体及其对灰霉病的生防活性评价。
J Microbiol. 2013 Apr;51(2):166-73. doi: 10.1007/s12275-013-2321-8. Epub 2013 Apr 27.
9
Cloning and characterization of a chitinase (chit42) cDNA from the mycoparasitic fungus Trichoderma harzianum.哈茨木霉几丁质酶（chit42）cDNA的克隆与特性分析
Curr Genet. 1994 Dec;27(1):83-9. doi: 10.1007/BF00326583.
10
Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli.哈茨木霉两种几丁质酶Chit33和Chit42在大肠杆菌中的异源表达及定点诱变研究
Protein Expr Purif. 2007 Feb;51(2):216-26. doi: 10.1016/j.pep.2006.07.020. Epub 2006 Aug 2.

引用本文的文献

1
Genome-wide characterization and expression analysis of the chitinase gene family in chickpea (Cicer arietinum L.) for fungal stress resistance.鹰嘴豆（Cicer arietinum L.）几丁质酶基因家族的全基因组特征分析及表达分析，以研究其对真菌胁迫的抗性
Mol Biol Rep. 2025 Sep 5;52(1):871. doi: 10.1007/s11033-025-10937-x.
2
Involvement of Pathogenesis-Related Proteins and Their Roles in Abiotic Stress Responses in Plants.病程相关蛋白的参与及其在植物非生物胁迫响应中的作用
Biomolecules. 2025 Jul 30;15(8):1103. doi: 10.3390/biom15081103.
3
Genome-Wide Identification and Expression Analysis of the GH19 Chitinase Gene Family in Sea Island Cotton.海岛棉中GH19几丁质酶基因家族的全基因组鉴定与表达分析
Curr Issues Mol Biol. 2025 Aug 7;47(8):633. doi: 10.3390/cimb47080633.
4
Genome-wide identification of chitinase gene family in Hordeum vulgare: insights into stress response mechanisms and evolutionary dynamics.大麦几丁质酶基因家族的全基因组鉴定：对胁迫响应机制和进化动态的见解
BMC Plant Biol. 2025 May 13;25(1):628. doi: 10.1186/s12870-025-06475-0.
5
Expression of endochitinase and exochitinase in lettuce chloroplasts increases plant biomass and kills fungal pathogen Candida albicans.生菜叶绿体中内切几丁质酶和外切几丁质酶的表达增加了植物生物量并杀死了真菌病原体白色念珠菌。
Plant Biotechnol J. 2025 May;23(5):1437-1451. doi: 10.1111/pbi.14596. Epub 2025 Feb 18.
6
Comparative Long Non-Coding Transcriptome Analysis of Three Contrasting Barley Varieties in Response to Aluminum Stress.三种不同耐铝性大麦品种响应铝胁迫的长链非编码转录组比较分析。
Int J Mol Sci. 2024 Aug 23;25(17):9181. doi: 10.3390/ijms25179181.
7
Cloning of maize chitinase 1 gene and its expression in genetically transformed rice to confer resistance against rice blast caused by Pyricularia oryzae.克隆玉米几丁质酶 1 基因并在遗传转化水稻中表达以赋予对稻瘟病菌引起的稻瘟病的抗性。
PLoS One. 2024 Jan 16;19(1):e0291939. doi: 10.1371/journal.pone.0291939. eCollection 2024.
8
Genome-Wide Identification and Expression Analysis of Chitinase Genes in Watermelon under Abiotic Stimuli and Infection.在非生物胁迫和真菌感染下西瓜几丁质酶基因的全基因组鉴定和表达分析
Int J Mol Sci. 2024 Jan 4;25(1):638. doi: 10.3390/ijms25010638.
9
Cloning and Expression of Class I Chitinase Genes from Four Mangrove Species under Heavy Metal Stress.重金属胁迫下四种红树植物I类几丁质酶基因的克隆与表达
Plants (Basel). 2023 Jul 26;12(15):2772. doi: 10.3390/plants12152772.
10
Overexpression of chitinase from improves broad-spectrum disease resistance of Arabidopsis.几丁质酶 3 基因的过量表达提高了拟南芥的广谱抗病性。
Virulence. 2023 Dec;14(1):2233147. doi: 10.1080/21505594.2023.2233147.

本文引用的文献

1
Increased Antifungal Activity of Trichoderma harzianum Transformants That Overexpress a 33-kDa Chitinase.哈茨木霉转化株过表达 33kDa 几丁质酶后抗真菌活性增强。
Phytopathology. 1999 Mar;89(3):254-61. doi: 10.1094/PHYTO.1999.89.3.254.
2
A simple and general method for transferring genes into plants.一种将基因转入植物的简单而通用的方法。
Science. 1985 Mar 8;227(4691):1229-31. doi: 10.1126/science.227.4691.1229.
3
Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis.辣椒II类碱性几丁质酶基因CAChi2的启动子激活以及过表达CAChi2的拟南芥对细菌性疾病抗性和渗透胁迫耐受性的增强。
Planta. 2006 Feb;223(3):433-48. doi: 10.1007/s00425-005-0099-6. Epub 2005 Sep 6.
4
Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses.在转基因烟草中的过表达揭示了水稻同源异型域基因OsBIHD1在生物和非生物胁迫响应中的不同作用。
J Exp Bot. 2005 Oct;56(420):2673-82. doi: 10.1093/jxb/eri260. Epub 2005 Aug 16.
5
The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants.细菌延伸因子Tu的N端在拟南芥植物中引发先天免疫。
Plant Cell. 2004 Dec;16(12):3496-507. doi: 10.1105/tpc.104.026765. Epub 2004 Nov 17.
6
Pathogenesis-related gene expression by specific calmodulin isoforms is dependent on NIM1, a key regulator of systemic acquired resistance.特定钙调蛋白亚型的病程相关基因表达依赖于NIM1，NIM1是系统获得性抗性的关键调节因子。
Mol Cells. 2004 Oct 31;18(2):207-13.
7
Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.在一个过表达病程相关（PR）蛋白基因的转基因小麦品系中，损伤模拟表型的发育取决于水杨酸浓度。
Mol Plant Microbe Interact. 2003 Oct;16(10):916-25. doi: 10.1094/MPMI.2003.16.10.916.
8
Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding.解析植物与病原体的交流：分子抗性育种的新视角
Curr Opin Biotechnol. 2003 Apr;14(2):177-93. doi: 10.1016/s0958-1669(03)00035-1.
9
Molecular biology and application of plant peroxidase genes.植物过氧化物酶基因的分子生物学及其应用
Appl Microbiol Biotechnol. 2003 Feb;60(6):665-70. doi: 10.1007/s00253-002-1157-7. Epub 2002 Dec 18.
10
Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase.水稻中的抗病性和非生物胁迫耐受性受到一种脱落酸诱导的丝裂原活化蛋白激酶的反向调控。
Plant Cell. 2003 Mar;15(3):745-59. doi: 10.1105/tpc.008714.