过量表达真菌来源几丁质酶的转基因烟草植株对生物和非生物胁迫因子表现出增强的抗性。
Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents.
作者信息
de las Mercedes Dana María, Pintor-Toro José A, Cubero Beatriz
机构信息
Instituto de Recursos Naturales y Agrobiología de Sevilla, Consejo Superior de Investigaciones Científicas, 41012-Seville, Spain.
出版信息
Plant Physiol. 2006 Oct;142(2):722-30. doi: 10.1104/pp.106.086140. Epub 2006 Aug 4.
Abstract
Genes encoding defense-related proteins have been used to alter the resistance of plants to pathogens and other environmental challenges, but no single fungal gene overexpression has produced broad-spectrum stress resistance in transgenic lines. We have generated transgenic tobacco (Nicotiana tabacum) lines that overexpress the endochitinases CHIT33 and CHIT42 from the mycoparasitic fungus Trichoderma harzianum and have evaluated their tolerance to biotic and abiotic stress. Both CHIT33 and CHIT42, individually, conferred broad resistance to fungal and bacterial pathogens, salinity, and heavy metals. Such broad-range protective effects came off with no obvious detrimental effect on the growth of tobacco plants.
摘要
编码防御相关蛋白的基因已被用于改变植物对病原体和其他环境挑战的抗性，但在转基因品系中，单一真菌基因的过表达并未产生广谱的胁迫抗性。我们构建了过表达来自哈茨木霉的内切几丁质酶CHIT33和CHIT42的转基因烟草（烟草）品系，并评估了它们对生物和非生物胁迫的耐受性。单独的CHIT33和CHIT42均赋予了对真菌和细菌病原体、盐度和重金属的广泛抗性。这种广泛的保护作用并未对烟草植株的生长产生明显的不利影响。
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